[目的]为鸡腿菇多糖的生产提供有益的参考,拓宽鸡腿菇的食品应用价值.[方法]在单因素试验的基础上进行正交试验,确定酶解法提取鸡腿菇多糖的最佳工艺条件.[结果]温度、pH和水解时间3个因素对酶解法提取鸡腿菇多糖的影响依次为温度>水解时间>pH,但差别不大.鸡腿菇多糖提取的最佳工艺条件为:水解时间3.5h,温度30℃,pH3.0,纤维素酶1.0%.在该最佳方案下,鸡腿菇多糖提取率为17.1%.[结论]优化了酶解法提取鸡腿菇多糖的工艺.%[Objective] The useful reference for the production of polysaccharides from Coprinus comatus was provided through the research, which would widen its application value in food industry. [ Method ] The optimum condition of the polysaccharide extracted from Coprinus comatus with the method of hydrolysate was determined through the orthogonal experiment based on the single factor test. [ Result] The result indicated that the order of the factor affecting its extracting efficiency was temperature > time > pH value and the optimum condition was the time was 3.5 hours, the temperature was 30℃ , the pH value was 3.0 and the application amount of cellulose was1. 0% , under which condition, the extracting rate of polysaccharides was 17.4%. [ Conclusion] The method of polysaccharide extracted from Coprinus comatus was optimized.
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