[Objective] The aim was to establish a determination method for the catechin content of RHIZOMA BERGENIAE SCOPULOSAE by HPLC. [Method] Ultrasonic-assisted extraction was adopted and HPLC was used for the determination. The chromatographic conditions were:Kromasil-C,,(4.6 mm x 150 mm,5 jun ) as the column,methanol-water-glacial acetic acid ( V/V/V, 15:84:1) as the mobile phase, 1.0 ml/min flow rate,and 276 nm detection wavelength. [ Result] The linear range of catechins was between 0. 624 and 3. 12 (tg/ml(r =0.999 3) ;the average recovery was 100.05% ,with RSD = 1.73% (n =6). [Conclusion] This method was simple and accurate,and could be used for the determination of xatechins in RHIZOMA BERGENIAE SCOPULOSAE.%[目的]建立HPLC测定盘龙七中儿茶素含量的方法.[方法]采用超声波辅助提取,HPLC法测定,色谱条件为:色谱柱为Kromasil-C18(4.6 mm× 150 mm,5μm),流动相为无水甲醇-水-冰醋酸(15:84:1,V/V/V),流速为1.0 ml/min,检测波长为276 nm.[结果]儿茶素的线性范围为0.624 ～3.12 μg/ml(r =0.999 3),平均回收率为100.05％,RSD为1.73％(n=6).[结论]该测定方法简便、准确,可用于盘龙七中儿茶素的含量测定.
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