[Objective] The aim was to test and verify maturation protein of bovine myostatin(MSTN) gene based on prokaryotic expression,to provide basis for inoculation experiment of mice. [ Method] RT-PCR method was used to amplify coding sequence of maturation protein of bovine myostatin(MSTN) gene, then after double digestion, amplification products were cloned to expression vector pET28a( + ), transform escherichia coli BL21, after induction of IPTG, SDS-PAGE and Western-blot were used to detect expression of results of recombinant protein.[Result] Anti-His-Tag identification was correct. [Conclusion] Beef cattle MSTN were expressed in prokaryotic expression system correctly.%[目的]原核表达肉牛肌生成抑制素成熟蛋白编码序列基因并进行功能验证,为后期的小鼠接种实验打下基础.[方法]采用RTPCR方法扩增肉牛MSTN成熟蛋白编码序列基因,该扩增产物经过双酶切后克隆到表达载体pET28a(+)中,转化大肠杆菌BL21,经IPTG诱导表达后采用SDS-PAGE和WesteRN-blot检测重组目的蛋白的表达结果.[结果]经Anti-His-Tag鉴定正确.[结论]肉牛MSTN 成熟蛋白编码序列基因在原核表达系统中得到正确表达.
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