[Objective] The aim is to explore antioxidation of kiwi fruit root extract in vitro. [ Method] Using DPPH free radicals, superoxide, hydroxyl radicals, hydrogen peroxide and reduction force reaction system to determine antioxidation of kiwi fruit root extract in vitro and comparing with vitamin C experiments. [ Result] Under experimental conditions, the scavenging or inhibition of ERHM on DPPH free radicals, superoxide anion (O-2 · ) , hydroxyl radical (·OH) and H2O2 are stronger, and show good dose-effect relationship, also has some reducing power. The EC50 for the elimination of DPPH radical is 8.03 μg/ml and for superoxide anion (O-2 ·) is 1.28 mg/ml, inhibition of hydroxyl radical (·OH) capacity is up to 69.4% , when the concentration is 100 μg/ml, the clearance rate of hydrogen peroxide ( H2O2) is 42%. [ Conclusion] Kiwi root extract has strong reducing power, can effectively remove the DPPH and superoxide anion radicals, and inhibit the production of hydroxyl radicals. Therefore, ERHM active ingredient has the more significant antioxidant activity.%[目的]探讨猕猴桃根提取物的体外抗氧化作用.[方法]采用DPPH自由基、超氧阴离子、羟基自由基、过氧化氢和还原力反应体系,测定猕猴桃根提取物的体外抗氧化作用,并用维生素C进行对照试验.[结果]试验条件下,ERHM对DPPH自由基、超氧阴离子(O-2·)、羟基自由基(·OH)、过氧化氢(H2O2)等均有较强的清除或抑制作用,且显示较好的量效关系,同时具有一定的还原力.其消除DPPH自由基的EC50为8.03 μg/ml,清除超氧阴离子(O-2·)的EC50为1.28 mg/ml,抑制羟基自由基(·OH)能力可达69.4％,浓度为100μg/ml时,对过氧化氢(H2O2)的清除率为42％.[结论]猕猴桃根提取物具有较强的还原力,能有效清除DPPH和超氧阴离子自由基,并抑制羟基自由基的产生.所以,ERHM有效成分具有较为显著的抗氧化作用.
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