[目的]分析评价杂交鳢及亲本对病原菌的抗病力水平.[方法]通过人工感染试验方法,开展了杂交鳢(乌鳢♂×斑鳢♀)及其亲本对两种病原菌的抗感染试验.[结果]以嗜水气单胞菌为试验菌株、菌液浓度9 × 108 cfu/ml(100、10-1、10-2、10-34个稀释度)、注射剂量0.5ml时,3d内杂交鳢LD50平均值介于乌鳢与斑鳢间,且差异均不显著;以诺卡氏菌为试验菌株、菌液浓度7×109 cfu/ml( 100、10-1、10-2、10-34个稀释度)、注射剂量0.5ml时,30d内杂交鳢LD50平均值介于斑鳢与乌鳢间,且差异也均不显著.[结论]高浓度感染时,嗜水气单胞菌、诺卡氏菌对3种鳢鱼均有极强的致病性,108cfu/ml菌液浓度时,3种鳢鱼死亡率达100%;不过低浓度感染时致死率明显减弱,且不同品种对病原菌的耐受力有些差异.%[Objective] The study aimed to analyze and evaluation the anti-infective level of to the pathogenic bacteria. [ Method] The test of anti-infection to 2 pathogenic bacteria was conducted on the hybrid snakehead and its parents through the artificial infection test method. [ Results] When Aeromonas hydrophila was the tested strain, the bacterium fluid was 9 × 108 cfu/ml in which there were 4 dilution grade of 100, 10 -1,10-2 and 10-3,the injection dosage was 0.5 ml/fish, the median lethal dose (LD50) on the hybrid snakehead was between C. Argus and C. Maculate,and no significant difference was found among the strains. When Nocardia seriolea was the tested strain ,the bacterium fluid was 7 ×109 cfu/ml in which there were 4 dilution grade of 100, 10-1 ,10-2 and 10-3, the injection dosage was 0.5 ml/fish, the median lethal dose (LD50) on hybrid snakehead was between C. Maculate and C. Argus,and no significant difference was found among the strains. [Conclusion] When the bacterium fluid was at high levels of infection, both A. Hydrophila and Nocardia seriolea were highly pathogenic to 3 kinds of the snakehead fish. When the bacterium fluid was 108 cfu/ml, the mortality rate of the snakehead fish was 100%. When the fluid was at low level of infection, the mortality rate was significantly weakened. The different varieties had some different tolerance to the pathogenic bacteria.
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机译:杂交鳢(乌鳢♂ × 斑鳢♀)及其亲本血清胆红素和尿素氮的比较分析 Comparative Analysis on the Serum Bilirubin and BUN of Hybrid Snakehead (Channa Argus♂ × Channa Maculate♀) and Its Parents