[Objective] This study was to screen out suitable genotypes and basic medium for the culture of maize mature embryos. [ Method] Using mature embryos of nine maize genotypes as explants, the effects of genotypes and basic medium on callus induction and subculture were investigated. [Result] The genotypes performed better in callus induction and subculture were found in turn 853-35, 853-209, Dan 34 and 81162. MS medium is better than N6 medium in the callus induction from maize embryos, while N6 medium is more suitable for callus subculture. [Conclusion] Our study further improved the tissue culture system in maize with mature embryos as explants.%[目的]筛选出适宜玉米成熟胚组织培养的基因型和基本培养基.[方法]以9个基因型的玉米成熟胚为外植体,研究基因型与基本培养基对成熟胚愈伤组织诱导和继代的影响.[结果]试验中表现较好的基因型依次是853-35、853-209、丹34和81162;对于玉米成熟胚愈伤组织的诱导培养,MS培养基比N6培养基更适宜,而对于玉米成熟胚愈伤组织的继代培养,N6的培养基比MS培养基更适宜.[结论]进一步完善了以玉米成熟胚为外植体的组织培养体系.
展开▼
