[Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis. [ Method] The endogenous NO level in roots of wild-type Arabidopsis thaliana and NahG mutant growing at 4 ℃ was measured with NO-specific probe DAF-FM DA staining method. [Result] NO level in plants growing at 4 X. For a long term could be increased with time; low temperature could induce NO production in wild-type and NahG mutants of A. Thaliana, in which the NO level in NahG mutant with low level of salicylic acid was significantly lower than that in wild-type. [Conclusion] NO was involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis at low temperature.%[目的]验证NO是否参与了水杨酸对拟南芥生长的抑制过程,为植物在长期低温下生长的分子机制研究奠定基础.[方法]用可渗透细胞膜的NO特异性探针DAF-FM DA染色法测定4℃低温处理下,野生型拟南芥(Arabidopsis thaliana)和NahG突变体根中内源NO含量的变化.[结果]长期低温4℃处理可诱导植物中NO含量增加,并随时间的延长而升高；低温能够诱导野生型和NahG突变体中NO的产生,但体内水杨酸含量低的NahG突变体中NO含量明显低于比野生型.[结论]低温条件下NO参与了水杨酸对拟南芥生长的抑制过程.
展开▼
