首页> 中文期刊> 《安徽农业科学》 >节瓜枯萎病生防菌对杀虫剂的适应性研究

节瓜枯萎病生防菌对杀虫剂的适应性研究

         

摘要

[ Objective] The theoretical basis of the optimal preparation of antagonistic bacteria and pesticides,and the improvement of the controlling effect of the fusarium wilt( Fusarium oxysporum Schl. f. sp . benincasa) of Benincasa hispida Coga. var. chich-qua H. was provided through the adaptability of the Bio-control bacteria controlling the fusarium wilt of Benincasa hispida Coga. var. chich-qua H. to the pesticide. [ Method] The inhibitory role of the mixture of 3 Bio-control bacteria;Bio-A1 ,Bio-B22 and Bio-D10,and the insecticide:thiazole phosphate,chlorpyrifos and phoximed,respectively,to the spore germination of the fusarium wilt of Benincasa hispida Coga. var. chich-qua H. was determined; and also,the effect of the insecticide on the growth and reproduction and colony morphology of the Bio-control bacteria controlling the fusarium wilt of Benincasa hispida Coga. var. chich-qua H. was analyzed after the 3 Bio-control bacteria;Bio-A1 ,Bio-B22 and Bio-D10 were inoculated/cultured in the fermentation solution containing thiazole phosphate .chlorpyrifos and phoximed. [ Results] Hie results showed that the Bio-control bacteria Bio-Al, Bio-B22 and the Bio-D10 were with the good adaptability in the insecticides. The antibacterial activity of thiazole phosphate could significantly improve the activity of Bio-B22 and the Bio-D10. Three insecticides did not show the effect on the colony morphology of three Bio-control bacteria. The thiazole phosphate did not affect the reproduction of Bio-D10. However,Three insecticides could significantly reduce the reproductive number of Bio-Al and Bio-B22. [Conclusion] The efficiency of the Bio-control bacteria mixed with the insecticides was not reduced%[目的]研究节瓜枯萎病生防菌对常用杀虫荆的适应性,为配制拮抗细菌和杀虫剂的最佳复合制剂、提高节瓜枯萎病的防治效果提供理论依据.[方法]将3株节瓜枯萎病生防细茵Bio-A1、Bio-B22和Bio桪10的发酵液分别与杀虫剂福气多、乐斯本、绿色金通混合.测定其混合液对节瓜枯萎病病菌孢子萌发的抑制作用;将3株节瓜枯萎病生防细菌Bio-A1、Bio-B22和Bio-D10与接种在舍有杀虫剂福气多、乐斯本、绿色金通的培养液中发酵,测定杀虫剂时拮抗菌生长繁殖及其茵落形态的影响.[结果]拮抗菌Bio-A1、Bio-B22和Bio-D10对这3种杀剂均能很好地适应,福气多还可显著提高拮抗菌Bio-B22和Bi0-D10的抑菌活性;福气多、绿色金通和乐斯本对3种括抗细菌Bio-A1、Bio-B22和Bio桪10的菌落形态没有影响,福气多对拮抗菌Bio-D10的繁殖没有影响;这3种药剂能显著地减少桔抗细菌Bio桝1和Bio-B22的繁殖数量.[结论]生防菌发酵液与杀虫刑混合后,不会影响其抑菌活性,完全可以混合使用.

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