[ Objective] The aim of the study was to provide basic date for biochemical genetic study of Qinghai Alectoris graeca. [ Method] 39 Qinghai Alectoris graeca were used to study isozyme pattern of serum amylase and activity with vertical polypropylene gel electrophoresis and spec-trophotometry.respectively. [Result] The study showed that there were two isozymes of serum amylase including Amy-1 and Amy-3 in Qinghai Alectoris graeca. TTiere were three kinds of types that were AA,AB and BB in Amy-1 i&ozyme ,and the phenotype frequency of AA, AB and BB were 17.95% ,66.67% ,15.38%. The AB was the advantageous type, and the allele frequency of Amy-1~A and Amy-1~B were 0.5128 and 0.4872 respectively. The gene heterozygosity was 0.499 7. Tlie average activity of serum amylase was (566.62±112,20)U/L. [Conclusion] The Amy-1 isozyme existed the polymorphism in Qinghai Alectoris graeca. The activity of serum amylase in the Qinghai Alectoris graeca with Amy-1 AB phenotype was significantly higher than that in the Alectoris graeca with Amy-1 AA and Amy-1 BB phenotype.%[目的]为青海石鸡的生化遗传学研究提供基础资料.[方法]采用聚丙烯酰胺凝胶垂直平板电泳法对39只青海石鸡血清淀粉酶同工酶酶谱进行研究,并利用比色法对其淀粉酶活性进行测定.[结果]青海石鸡血清淀粉酶有Amy-1,Amy-3 2种同工酶, 其中Amy-1有AA、AB和BB 3种表型,表型频率分别为17.95%、66.67%、15.38%;AB为优势表型,Amy-1~A和amy-1~B等位基因频率分别为0.512 8和0.487 2,基因杂合度为0.499 7;青海石鸡血清淀粉酶活性为(566.62±112.20)U/L.[结论]青海石鸡Amy-1同工酶呈现多态性,Amy-1 AB表型的血清淀粉酶活性显著高于Amy-1 AA和Amy-1 BB表型.
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