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首页> 外文期刊>Biotechnology Progress >Reactivity of pure Candida rugosa lipase isoenzymes (Lip1, Lip2, and Lip3) in aqueous and organic media. Influence of the isoenzymatic profile on the lipase performance in organic media
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Reactivity of pure Candida rugosa lipase isoenzymes (Lip1, Lip2, and Lip3) in aqueous and organic media. Influence of the isoenzymatic profile on the lipase performance in organic media

机译：纯的假丝酵母念珠菌脂肪酶同工酶（Lip1，Lip2和Lip3）在水和有机介质中的反应性。同工酶谱对有机介质中脂肪酶性能的影响

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摘要

Three pure isoenzymes from Candida rugosa lipase (CRL: Lip1, Lip2, and Lip3) were compared in terms of their stability and reactivity in both aqueous and organic media. The combined effect of temperature and pH on their stability was studied applying a factorial design. The analysis of the response surfaces indicated that Lip1 and Lip3 have a similar stability, lower than that of Lip2. In aqueous media, Lip3 was the most active enzyme on the hydrolysis of p-nitrophenyl esters, whereas Lip1 showed the highest activity on the hydrolysis of most assayed triacylglycerides. The highest differences among isoenzymes were found in the hydrolysis of triacylglycerides. Thus, a short, medium, and long acyl chain triacylglyceride was the preferred substrate for Lip3, Lip1, and Lip2, respectively. In organic medium, Lip3 and Lip1 provided excellent results in terms of enantioselectivity in the resolution of ibuprofen (EF value over 0.90) and conversion, whereas initial esterification. rate was higher for Lip3. However, the use of Lip2 resulted in lower values of conversion, enantiomeric excess, and enantioselectivity. In the case of trans-2-phenyl-1-cyclohexanol (TPCH) resolution, initial esterification rates were high except for Lip3, which also produced poor results in conversion and enantiomeric excess. The performance of the pure isoenzymes in the enantioselectivity esterification. of these substrates was compared with different CRL crude preparations with known isoenzymatic content and the different results could not be explained by their isoenzymatic profile. Therefore, it can be concluded that other factors can also affect the catalysis of CRL and only the reproducibility between powders can ensure the reproducibility in synthesis reactions.
机译：比较了来自假丝酵母念珠菌脂肪酶的三种纯同工酶（CRL：Lip1，Lip2和Lip3）在水性和有机介质中的稳定性和反应性。应用析因设计研究了温度和pH对其稳定性的综合影响。响应面的分析表明，Lip1和Lip3具有相似的稳定性，但低于Lip2。在水性介质中，Lip3是对硝基苯酯水解的最活跃酶，而Lip1在大多数测定的三酰基甘油酯的水解中表现出最高的活性。在三酰基甘油酯的水解中发现同工酶之间的差异最大。因此，短，中和长酰基链甘油三酯分别是Lip3，Lip1和Lip2的首选底物。在有机介质中，Lip3和Lip1在布洛芬的拆分（EF值超过0.90）和转化率（初始酯化）的对映选择性方面提供了出色的结果。 Lip3的使用率更高。但是，使用Lip2会导致较低的转化率，对映体过量和对映选择性。在反式-2-苯基-1-环己醇（TPCH）的情况下，除Lip3以外，其初始酯化率很高，而Lip3的转化率和对映体过量也产生不良结果。纯同工酶在对映选择性酯化反应中的性能。将这些底物的样品与具有已知同工酶含量的不同CRL粗制品进行了比较，其同工酶谱无法解释不同的结果。因此，可以得出结论，其他因素也可以影响CRL的催化，只有粉末之间的可再现性才能确保合成反应中的可再现性。

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