[目的]实现楸树离体的快速繁殖,并为楸树的大规模种苗生产提供理论依据.[方法]以楸树腋芽为外植体,通过选择不同培养基和激素配比,研究楸树组培及快繁技术.[结果]筛选出效果较好的培养基,分别为腋芽萌发:N_6+6-BA 2.0 mg/L+NAA 0.005 mg/L;愈伤组织诱导:MS+6-BA 1.5 mg/L+NAA 2.0 mg/L;芽分化:MS+6-BA 0.2～0.5 mg/L+NAA 0.5～1.0 mg/L;继代增殖:N_6+6-BA 1.0～1.5 mg/L+NAA 0.005～0.01 mg/L;生根培养:1/2 MS+NAA 0.5～1.0 mg/L+活性碳1g/L.[结论]用组织培养法快速繁殖楸树,能大大提高楸树的繁育速度.%[Objective] The aim was to achieve rapid propagation of Catalpa bungei and provide a theoretical basis for large-scale seedling production. [Method] Axillary bud of Catalpa bungei were used as explants, while different medium and hormone combinations were selected to set up the tissue culture and rapid propagation system of Catalpa bungei . [Result] N_6+2.0 mg/L 6-BA +0.005 mg/L NAA is an appropriate culture medium for germination of axillary bud, MS+1.5 mg/L 6-BA +2.0 mg/L NAA is the best medium for callus induction, MS+0.5 mg/L 6-BA +0.5 mg/L NAA is suitable for bud differentiatation and N_6+1.5 mg/L 6-BA +0.005 mg/L NAA for subculture multiplication. 1/2 MS+0.5 mg/L NAA was of advantage to rooting culture. [Conclusion] The propagation speed of Catalpa bungei was greatly improved by tissue culture method.
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