PGIP gene was obtained from Brassica oleracea L. var. alboglabra ,named BoPGIP2 . The full length of BoPGIP2 gene is 1 102 bp and the exon is 993 bp which encodes a protein of 330 amino acids with a predicted molecular mass of 37.1 kDa, interrupted by one intron of 95 bp in length. Sequence analysis revealed that it has five potential N-glycosylation sites, two protein kinase C phosphrylation sites, five casin kinase II phosphrylation sites and four N-myristoylation sites. The amino acids sequences alignment confirmed that ~(145) LRR stucture was highly conserved in all aligned PGIP sequences.%从芥蓝 (Brassica oleracea L.var.alboglabra)中克隆了一个新的PGIP基因,命名为BoPGIP2.BoPGIP2基因序列全长为1 102 bp,由2个外显子和1个内含子构成,外显子总长为993 bp,内含子总长为95 bp,编码330个氨基酸序列,分子量为37.1 kDa.推导的氨基酸序列分析表明,该序列包含5个N-糖基化位点,2个蛋白激酶C磷酸化位点,5个酪蛋白激酶II磷酸化位点,4个N-豆蔻酰化位点;同源序列比对分析表明,所比对基因都含有LRR结构,在145位点的LRR结构域在所有物种中高度保守.
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