[目的]建立利用量子点增强鲁米诺-过氧化氢-铁(III)化学发光体系测定铁的新方法.[方法]利用CdTe量子点和鲁米诺-过氧化氢-铁(Ⅲ)化学发光体系对样品溶液中的铁含量进行了测定.[结果]单因素试验表明,量子点强烈地增强了化学发光信号,从而增加了反应体系的灵敏度;当鲁米诺浓度为1.0×10~(-4) mol/L,量子点浓度为7%,过氧化氢浓度为0.5%时,化学发光信号最强.在10～700 ng/ml的浓度范围内,铁的浓度与化学发光强度呈良好的线性关系,其线性回归方程为ΔI=2.569C+212.7,检出限为2.25 ng/ml,RSD为2.6%.利用该法测得的血清1、2和水样1、2的铁含量分别为143.97、406.05、18.67、16.08 ng/ml.[结论]该法可用于测定人血清和自来水中的铁含量.%[Objective] The aim was to establish the new method of determining iron with CdTe quantum-dots intensified luminol-H_2O_2-Fe(III) chemiluminescence system. [Method] The iron content in sample solution was determined by using of the enhanced chemiluminescence system of luminol-H_2O_2-Fe(III) with CdTe quantum-dots. [Result] The single factor experiments showed that the chemiluminescence signals were intensified by quantum-dots intensively, so the sensitivity of reaction system was increased; when the luminol concn. was 1.0×10~(-4) mol/L, the quantum-dots concn. was 7%, and the concn. of H_2O_2 was 0.5%, the chemiluminescence signals were most intensive. The chemiluminescence intensity was linear with iron concn. in the range of 10-700 ng/ml and the linear regression equation was Δ I=2.569C +212.7, the detection limit was 2.25 ng/ml and the RSD was 2.6%. The iron contents in serum 1, 2 and water sample 1, 2 determined by this method were 143.97, 406.05, 18.67 and 16.08 ng/ml resp. [Conclusion] This method could be used to determine the iron contents in human serum and tap water.
展开▼
