目的：探讨电针干预对早期阿尔茨海默病小鼠脑组织间液( brain interstitial fluid,ISF)及海马组织中可溶性Aβ1-42水平的影响.方法将16只雄性5月龄APP/PS1转基因阳性小鼠随机分为模型组和针刺组,将8只同窝、同性别转基因阴性小鼠设为正常对照组,采用电针“百会”、“涌泉”或同等条件束缚作为干预手段,以脑微透析技术获取小鼠海马组织间液,应用ELISA法检测3组小鼠海马组织以及海马ISFβ淀粉样蛋白1-42水平,分别在干预后的6,7,8,9 h观察其是否发生改变.结果与正常对照组相比,模型组海马组织以及 ISF中可溶性Aβ1-42明显升高(P<0.01)；与模型组相比,针刺组小鼠ISF可溶性Aβ1-42降低(P<0.05),海马组织Aβ1-42水平对比差异无统计学意义(P>0.05).针刺组在电针治疗后6~9 h样品ISF中Aβ1-42含量的检测结果没有随时间变化而变化(P>0.05).结论电针可降低AD模型小鼠海马ISF可溶性Aβ1-42水平.%Objective To investigate the effects of electroacupuncture ( EA ) therapy on the levels of soluble Aβ1-42 in hippocampus and brain interstitial fluid ( ISF ) in mice with early-stage alzheimer ’ s disease ( AD ) . Method APP/PS1 double transgenic male mice with 5 months old were randomly divided into the model group and the acupuncture group. Wild type mice were used as the normal control group. Acupuncture on “Baihui”,“Yongquan” or constraint of the same condition was the intervention method, and the hippocampus interstitial fluid was obtained by cerebral microdialysis. The concentrations of Aβ1-42 in the hippocampus and ISF of the mice in three groups were measured by Elisa at the 6th, 7th, 8th and 9th hour after the intervention. Results Compared with the control group, the concentrations of Aβ1-42 in hippocampus and ISF of the model group were significantly higher (P<0. 01). Compared with the model group,the concentration of Aβ1-42 in ISF of the acupuncture group decreased (P<0. 05),but the concentrations showed no statistical difference between the two groups (P>0. 05). At the 6~9th hour after therapy,the concentration of Aβ1-42 of ISF in the treatment group did not change with time. Conclusion EA therapy can decrease the concentration of soluble Aβ1-42 in hippocampus ISF in mice with AD.
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