Objective To observe the inhibitory effect of oxaliplatin combined with baicalein on human gastric cancer cell line SGC-7901. Method Control group and drug-treated groups ( oxaliplatin group,baicalin group, and combined baicalin and oxaliplatin group) were set in this experiment. MTT assay was used to determine the effects of drugs at different concentrations on the proliferation of SGC-7901 cell line in the drug-treated groups;cell morphological changes of the cells cultured for 48 h in the control and drug-treated groups were observed under an inverted microscope;apoptosis rates of gastric carcinoma cell line SGC-7901 cells were detected by flow cytometry in the control and the drug-treated groups;HOE33258 staining was used to observe morphological changes of the apoptosis. Results The proliferation of SGC-7901 cells in oxaliplatin group,baicalein group,and combined baicalin and oxaliplatin groups could be effectively inhibited, showing an obvious dose-response relationship;the inhibition rate in the combined baicalin and oxaliplatin groups was significantly higher than that in oxaliplatin group and baicalin group,with significant differences (P<0. 01). The apoptosis rate of SGC-7901 cells in oxaliplatin group and baicalin group were significantly higher than that in the control group ( P<0 . 05 ) . The apoptosis rate in the combined baicalin and oxaliplatin groups was significantly higher than that in the control group,oxaliplatin group,and baicalin group (P<0. 05). Conclusion Oxaliplatin combined with baicalein can inhibit the proliferation of human gastric cancer cell line SGC-7901 cells and induce the cell apoptosis.%目的：观察奥沙利铂与黄芩素联用对人胃癌细胞株SGC-7901的抑制作用.方法实验分为对照组、给药组(奥沙利铂组、黄芩素组、联合作用组).应用MTT法测定给药组不同给药浓度对胃癌SGC-7901细胞株增殖的影响；倒置显微镜下观察对照组、给药组培养48 h后的细胞形态学变化；应用流式细胞术检测对照组、给药组胃癌SGC-7901细胞株凋亡率；HOE33258染色观察细胞凋亡形态学改变.结果奥沙利铂组、黄芩素组、联合作用组均可有效抑制SGC-7901细胞增殖，呈明显的量效关系；其中联合作用组抑制率明显高于奥沙利铂组和黄芩素组，差异具有显著统计学意义(P<0.01).奥沙利铂组、黄芩素组SGC-7901细胞凋亡率明显高于对照组，差异具有显著统计学意义(P<0.05).联合作用组细胞凋亡率明显高于对照组、奥沙利铂组、黄芩素组，差异具有显著统计学意义(P<0.05).结论奥沙利铂与黄芩素联用可显著抑制人胃癌细胞株SGC-7901细胞增殖，诱导细胞凋亡.
展开▼
