The isoflavone content of 8 different soybean samples was analyzed by HPLC/MS in this pa- per. The results showed that genistin(G) and malonygenistin (MG) are two major isoflavone species in all samples, and the content accounted more than 50% of total isoflavone content. The total content of Daizine (D) and malonygenistin (MD) ranked next to them, accounting about 38% of total isoflavone. Glycitin(GL) and malonyglycitin (MGL) had very low content in all samples, accounting about 6.8% of total isoflavone content. The big size soybean, such as yellow soybean, had higher total isoflavone content than both black soybeans and qing soybeans, while small size soybeans had higher total isoflavone content than big size soybeans. DPPH and FRAP results showed that black soybeans had higher antioxidant capa- bilities than yellow and qing soybean. And small size soybeans had higher antioxidant capabilities than big size soybeans.%利用液质联用及串联质谱法对黑豆、黄豆及青豆等8个大豆样品进行了大豆异黄酮分析,结果表明：染料木甙（G）及丙二酰基染料木甙（MG）在8个大豆样品中均为主要的大豆异黄酮组分,两者之和平均占到了大豆异黄酮总含量的50%以上;其次为大豆甙（D）和丙二酰基大豆甙（MD）,两者之和平均占大豆异黄酮总含量的38%左右;黄豆黄甙（GL）和丙二酰基黄豆黄甙（MGL）则在所有样品中含量均很低,两者之和平均只占总含量的6.8%.这8个样品中,对大粒种样品,黄豆中的大豆异黄酮含量高于黑豆及青豆,而小粒种大豆的异黄酮含量要明显高于大粒种.DPPH和FRAP抗氧化性分析结果则表明,黑豆的抗氧化性高于黄豆及青豆,而小粒种豆的抗氧化性高于大粒种豆.
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