[目的]探明蛋白质间的相互作用对于阐明细胞生命活动的分子机制具有重要意义.[方法]应用Cytoscape平台中MiMI插件,进行磷酸化修饰蛋白质之间的相互作用(protein-protein interaction,PPI)网络分析.采用基于DAVID分析系统对PPI网络中的蛋白质进行Gene Ontology (GO)富集分析.[结果]有5个磷酸化蛋白质(ATX1、AGL21、KNAT2、EOL2和VIP4)与其他33个蛋白质存在相互作用关系.这些PPI网络中的蛋白质主要参与分生组织的分生状态的维持,干细胞的发育以及基因的表达调控等生物过程.[结论]小立碗藓原生质体的再生机制可能与种子植物的胚后发育相似.%[Objective] Protein-protein interaction (PPI) determines the cell fate and is the focus of current research.It is important to elucidate the molecular mechanism of cell life activities.[Methods] Using MiMI plug of Cytoscape platform,PPI network was analyzed in Physcomitrella patens during protoplast regeneration.Gene Ontology (GO) annotations with DAVID system was analyzed for the proteins.[Results] Five proteins (ATX1,AGL21,KNAT2,EOL2 and VIP4) interacted with 33 proteins.Among them,the protein functions were mainly involved in maintenance of meristem identity,stem cell development,the regulation of gene expression and so on.[Conclusion] This study suggested that mechanism of protoplast regeneration from P.patens is similar to postembryonal development in seed plant.
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