Objective: To construct and identificate the siRNA reccmbinant plasmid of DNM1 and DNMT3 b genes.Methods: Four types of sENA reccmbinant plasmid of DNMT1 or DNMT3 b gene were constructed, and Hentificated. Results: After digested with Bam H Ⅰ andPst Ⅰ enzymes, all plasmids showed positive for reccmbinant vector plasmids. Two clones of each type ofvectorplasimid were selected for, sequencing identification. The results of sequencing identification were consistent with the inserted oligonucleotides Conclusions: The siRNA reccmbinant plasmds of DNMT1 and DNMT3 b genes were constructed corectly and could be used for further study.%目的:构建DNA甲基转移酶(DNMT1)、DNMT3b 微干扰RNA(siRNA)重组质粒,并鉴定其正确性.方法:构建DNMT1 siRNA重组质粒和DNMT3b siRNA重组质粒各4种,分别用BamH Ⅰ和Pst Ⅰ酶切、测序、鉴定.结果:用BamH Ⅰ和Pst Ⅰ进行双酶切后所有质粒均为阳性重组载体,每一种载体选择两个克隆进行测序鉴定,测序结果与插入的寡核苷酸的序列完全相符.结论:DNMT1 siRNA重组质粒和DNMT3b siRNA重组质粒正确,可用于后续实验.
展开▼
