Diethylaminoethyl(DEAE)-Agarose was studied for preparing monolayers based on the Langmuir-Blodgett(LB) film tech-nology .A high collapse pressure and a standard film forming process were found in the surface pressure (π)-area ( A) isotherm of DE-AE-Agarose.The effect of DEAE-Agarose concentration on the film formation showed that 0.5 mg/mL was the best .The surface poten-tial increased with rise of the ligand density .DEAE-Agarose LB films were characterized with Brewster angle microscopy ( BAM) and atomic force microscope ( AFM) after being transferred onto silicon surface .The morphology of the film was pretty compact , well struc-tured, and relatively smooth.The thickness was about 7.9 nm and the roughness was about 2 nm.This result indicated that the DEAE-Agarose LB film was successfully prepared and could be an novel material for the fabrication of LB films .%由于单分子膜性能独特，受到关注度越来越高，利用LB膜技术制备了二乙氨乙基（ DEAE）-琼脂糖单分子层。分析其表面压（π）－面积（ A）曲线，崩溃压较高，成膜过程明显。研究浓度对DEAE-琼脂糖成膜情况的影响，确定最佳成膜浓度为0.5 mg／mL。考察配基密度与表面电势的关系，其表面电势随着配基密度的增加而增大。采用原子力显微镜（AFM）和布鲁斯特角显微镜（BAM）对制备的LB膜进行了表征，得出表面膜的表面平整、紧密，厚度约为7.9 nm。 DEAE-琼脂糖LB膜制备成功，为以后LB膜技术的广泛应用提供了一种新的材料。
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