The toxicity effects of glufosinate ammonium to Tetrahymena thermophila were investigated from the viewpoints of cell prolif-eration and DNA damage .The growth curves of 96 hours were plotted by counting the cells every 12 hours;the tail length , tail moment and Olive tail moment of Tetrahymena thermophila were measured by Single Cell Gel Electrophoresis (SCGE).The results of growth curve test showed that there was a significant difference between drug groups and control group in cell density ( P<0.01 ) .According to SCGE data , the tail length , tail moment and Olive tail moment of the tested series were all significantly larger and higher than those in the control group ( P<0.01 ) , the DNA damage level was higher when toxic compound become heavier ( P<0.01 ) .The experi-mental concentration of glufosinate ammonium caused damages of cell DNA in varied extents , which reflects the acute and genetic toxic-ity of it to Tetrahymena thermophila .%从细胞增殖和DNA损伤角度研究不同浓度的草铵膦对嗜热四膜虫（ Tetrahymena thermophila）的毒性作用。采用12 h间隔细胞计数绘制96 h生长曲线，单细胞凝胶电泳技术检测受试四膜虫的尾长（ TL）、尾矩（ TM）及Olive尾矩（ OTM）。生长曲线绘制实验表明：草铵膦处理组与空白组种群细胞数目差异显著（ P＜0．01）。单细胞凝胶电泳实验表明：染毒各组的细胞TL、TM、OTM与对照组相比，均有显著增大（ P＜0．01） ，DNA损伤级别随着染毒浓度增大而升高（ P＜0．01）。实验浓度的草铵膦对细胞DNA均造成了不同程度的损伤，反映了其对四膜虫具有急性遗传毒性。
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