以青檀(Pteroceltis tatarinowii Maxim.)叶片为材料,采用正交设计和均匀设计两种方法对SRAP-PCR反应体系进行优化,并对这两种设计方案优化出的最佳反应体系进行比较,结果表明:两种设计均可用于青檀SRAP-PCR体系的优化,但与正交设计相比,均匀设计在多因素多水平条件下,得到的条带更清晰、稳定性更好.通过实验比较筛选出的青檀SRAP-PCR最佳反应体系为:2.5 μL 10×PCR buffer,20 ng模板DNA,Mg2+2.5 mmol/L,dNTP150 μmol/L,引物0.2μmol/L,Taq DNA聚合酶1.0 U,总体积25μL.%Orthogonal design and uniform design were used to obtain optimum scheme on reaction system for sequence-related amplified polymorphism (SRAP) of Pleroceltis latarinowii Maxim. , and the optimum reaction systems of the two designs were compared. The results showed that both orthogonal design and uniform design could be used for sequence-related amplified polymorphism of Pteroceltis tatarinowii. But compared with the orthogonal design, uniform design could quickly obtain the optimum reaction system for sequence-related amplified polymorphism of Pleroceltis tatarinowii with much clearer bands and better stability. The optimal 25 μL reaction system of SRAP for Pteroceltis tatarinowii included 2. 5 μL 10 × PCR buffer, 20 ng template DNA, Mg2 + 2. 5mmol/L, dNTP 150μmol/L, primer 0. 2μmol/L, Taq DNA polymerase 1. 0U.
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