Background] Cucumber green mottle mosaic virus ( CGMMV) is one of the most devastating pathogens of cucurbit crops. The virus has invaded more than ten provinces of China and infected crops such as watermelon and cucumber, causing serious eco-nomic losses. CGMMV infected watermelon and cucumber in Guangdong Province in 2009, but the resistance of cucumber and other cucurbits was unknomn. [Method] The resistance of 14 cucumber germplasms to a CGMMV isolate was tested using artificial me-chanical inoculation and nucleotide sequencing. [Result] The MP nucleotide sequence of CGMMV of Guangdong isolate from the diseased cucurbit samples was 99% similar to other isolates of CGMMV collected in China. All of 14 cucumber germplasms were sus-ceptible to the isolate of CGMMV. [Conclusion and significance] The main cucumber germplasms from Guangdong are not resistant to CGMMV. This result provides a scientific basis for control of CGMMV, and some guidance for cucumber breeding to resist CGM-MV in Guangdong.%【背景】黄瓜绿斑驳花叶病毒( Cucumber green mottle mosaic virus，CGMMV)是严重威胁葫芦科作物生产的毁灭性病原之一，该病毒已入侵我国十多个省份，危害西瓜、黄瓜等作物并造成严重的经济损失。早在2009年广东即发现CGMMV为害西瓜和黄瓜，但黄瓜等葫芦科作物对其抗性情况尚不清楚。【方法】采用人工机械摩擦接种方法，测定了14份黄瓜种质资源对CGMMV广东分离物的抗性水平。【结果】从广东葫芦病样中分离获得CGMMV，该病毒分离物MP基因序列与国内报道的各分离物同源率均在99％以上；14份黄瓜种质资源对该病毒分离物均表现为感病。【结论与意义】广东主要黄瓜资源对CGMMV均表现为感病，这为我省防控该病毒病提供了科学依据，也为黄瓜抗病育种提供了指导。
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