Objective To explore the possibility of hydroxyapatite/tricalcium phosphate(HA/TCP)matrix scaffold as cell carriers in tissue engineering,and to provide a direct evidence for the resurfacing of matrix scaffold. Methods Human bone marrow stromal cells (hBMSCs) and umbilical vein endothelial cells(UVECs) were co-cultured on the surface of matrix scaffold.The morphologic characters during culture on HA/TCP matrix scaffold′s surfaces were checked by light microscope,fluorescence microscope,and scanning electronic microscope.Results Both human bone marrow stromal cells and human umbilical vein endothelial cells showed good biocompatibility with HA/TCP matrix scaffold.The cell growth into the micropore of HA/TCP matrix scaffold could be seen under both fluorescence microscopy and scaning electron microscopy.Conclusion HA/TCP matrix scaffold can be used as a vehicle for the cell transplantation.%目的:探讨羟基磷灰石/磷酸三钙(HA/TCP)复合材料作为组织工程载体的可能;为基质材料表面改性提供实验基础.方法:应用人骨髓基质细胞(hBMSCs)和脐静脉内皮细胞(UVECs)和HA/TCP进行混合培养,应用光学显微镜、荧光显微镜及扫描电子显微镜对其进行观察.结果:人骨髓基质细胞和脐静脉内皮细胞在HA/TCP表面均生长良好,并可观察到细胞长入基质材料微孔内的改变.结论:羟基磷灰石/磷酸三钙(HA/TCP)复合材料可作为细胞移植的载体.
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