Objective To observe the effect of baicalin on RSA and explore its possible immune mechanism to regulate the maternal-fetal interface immune micro-environment.Methods SPF CBA/J females were randomly assigned to group A, B and C.The abortion rate and embryo absorption rate of each group were compared.The contents of CD4+, CD8+T lymphocytes, IFN-γand IL-4 were detected by immunohistochemistry and ELISA, and statistical analysis was performed.Results The abortion rate and embryo absorptivity of group C were significantly lower than those of group B (P＜0.01).The ratio of CD4+/CD8+T ratio and Th1/Th2 ratio in group C were similar to those in group A, but the difference was significant compared with group B (P＜0.05), the B group showed a higher CD4+/CD8+ratio and a Th1/Th2 ratio (P＜0.01).Conclusion Baicalin can inhibit the development of Th1/Th2balance and promote the development of Th2 type by regulating the immune state mechanism of uterus and maternal fetal interface, which can effectively reduce the ratio of CD4+/CD8+, and can effectively inhibit the occurrence of RSA.%目的观察黄芩苷对复发性流产(RSA)的影响,探索其陕西,宝鸡调节母胎界面免疫微环境的可能机制,为中药学"黄芩安胎"提供理论依据.方法分别将SPF级CBA/J雌鼠随机分为正常妊娠组(A)、复发性流产(RSA)对照组(B)和黄芩苷处理组(C).比较各组流产率、胚胎吸收率;经免疫组化、ELISA方法检测CD4+、CD8+T淋巴细胞、IFN-γ、IL-4的含量,并对比统计分析.结果C组较B组流产率及胚胎吸收率明显降低(P＜0.01);C组CD4+/CD8+T比值及Th1/Th2比值均与正常妊娠A组近似,但与B组相比差异具有显著性(P＜0.05),B组表现为CD4+/CD8+比值和Th1/Th2比值均升高(P＜0.01).结论黄芩苷通过调节子宫母胎界面免疫状态机制,促使Th1/Th2平衡偏向Th2型发展,明显有效降低CD4+/CD8+比值,能有效抑制复发性流产(RSA)行为的发生.
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