RP-HPLC fingerprint was established for proteins in goat milk powder to systematically evaluate goat milk quality via multicomponent analysis of milk proteins. Proteins from different goat milk were analyzed by (reversed-phase high-performance liquid chromatography) RP-HPLC. A chromatographic fingerprint similarity evaluation system and principal component analysis were utilized to correlate signals from different samples and identify milk quality. Seven caprine milk samples and two ovine milk samples were studied. The results show that major proteins including κ-CN, α-CN, β-CN, α-La and β-LgB from different samples have heterogeneous components and different retention times and mass fractions. Fingerprint similarity results show that the similarity among 6 caprine milk samples (G-3, G-4, G-5, G-6, G-8 and G-9) is 0.953~0.989, and the similarity among G-2 and the other 6 caprine milk samples is 0.721~0.832, while the similarity between the 2 ovine milk samples is 0.996. Principal component analysis indicates that the ovine and caprine milk samples can be classified into two categories and the difference can be easily identified. The proteins in the samples can be used as identification components to reflect the category and quality of goat milk powder. The method established in this paper is useful for quality evaluation and control of dairy products.%从研究羊乳品的质量出发,通过羊乳蛋白多组分信息分析,建立一种羊乳蛋白RP-HPLC指纹图谱评价方法,能够更完整和全面地评价羊乳的质量.利用 RP-HPLC 对不同来源的羊奶粉中的蛋白质进行分析,通过色谱指纹图谱相似度评价系统和主成分分析法,对乳蛋白 RP-HPLC 结果进行相关性评价,实现对乳源品质的鉴别.测定了7批山羊乳样品和2批绵羊乳样品,在不同样品中的主要蛋白质κ-CN、α-CN、β-CN、α-La、β-LgB出现不同的异质性组分、保留时间和质量分数.进行了指纹图谱相似度评价,结果表明,在7批山羊乳样品中,其中 G-3、G-4、G-5、G-6、G-8、G-9六批样品间的相似度在0.953~0.989,G-2样品与其他六批山羊乳样品的相似度在0.721~0.832;两批绵羊乳间的相似度在0.996.从主成分分析判断:绵羊、山羊两种乳样品可以归为两个大类,不同样品之间存在的差异能容易地被辨别出来.结果表明,羊乳蛋白质作为羊乳中的特征性成分,较好地反映了羊乳乳源的品类和质量信息.研究所建立的方法为有效地评价乳品的质量提供了极大的参考意义,也为企业建立特定羊乳乳源的指纹图谱,进行上下游加工的质量控制,成品的质量监测奠定基础.
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