基于微生物电解池构建了新型生化需氧量(BOD)快速测定生物传感器，以葡萄糖-谷氨酸溶液为模拟废水对传感器的性能进行了评估。结果表明：(1)当外加电压保持为0.7 V，传感器的最大电流与BOD浓度在10~400 mgL1内符合Monod方程，且传感器的最大电流和BOD浓度在10~100 mgL1呈线性关系；(2)传感器的测量时间短，BOD浓度在10~400 mgL1测量时间约为10 min；(3)传感器的重复性(±SD<±12.2%，n=6)和稳定性(±SD<±6%，12 d)好。结论：基于微生物电解池开发新型 BOD 生物传感器是可行的，且传感器具有灵敏度高、线性范围宽、检测时间短、重复性及稳定性好等优点，并能快速测定BOD。%A novel microbial electrolysis cell (MEC) based biosensor for rapid determination of biochemical oxygen demand (BOD) was developed, and its performance was evaluated with glucose-glutamic acid containing artificial wastewater. The results show that when the applied voltage is kept at 0.7 V, the maximum current of the biosensor follows Monod equation under BOD concentration of 10~400 mgL1, and the maximum current has linear relationship with BOD when the BOD concentration is in the range of 10~100 mgL1. The results also indicate that the measurement time is about 10 min when the BOD concentration is in the range of 10~400 mgL1. The relative standard deviation of repeatability was less than ±12.2%, while the relative standard deviation of stability was less than ±6% over a period of 12 days. These results demonstrate that the development of novel biosensors based on MEC for rapid determination of BOD is feasible, and the biosensor has advantages of high sensitivity, wide linear range, short detection time, good repeatability and good stability.
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