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The Use of the Method of Reduction of Methylene Blue for the Rapid Determination of the Living Bacteria Count when Growing the Plague Microbe by the Depth Method in the Reaction Tank

机译：利用反应池深度法生长鼠疫微生物快速测定亚甲蓝的方法快速测定活菌数

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There is a direct correspondence between the living microbe count determined by the cultural method, and the discoloration rate of methylene blue at individual phases of growth of the plague microbe in the reaction tank. The method of reduction of methylene blue can be used for the rapid determination of living microbes in the process of growth of the plague microbe in a reaction tank under the aeration conditions. The additional nutrition of the plague microbe with glucose in the process of growth of the plague microbe, which results in a sharp decrease of pH of the culture medium, disrupts the established direct correspondence between the discoloration rate of methylene blue and the living microbe count, determined by the cultural method. (Author)

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Liskina, I. V.;
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年度 1965
页码 p.1-8
总页数 8
原文格式 PDF
正文语种 eng
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关键词
Pasteurella pestis ; Counting methods ; Methylene blue ; Reduction(Chemistry) ; Growth ; Glucose ; Culture media ; Additives ; PH ; USSR;

机译：巴斯德氏菌;计数方法;亚甲蓝;还原（化学）;生长;葡萄糖;培养基;添加剂; pH;苏联;

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3. Competitive adsorption of methylene blue and brilliant green onto graphite oxide nano particle following: Derivative spectrophotometric and principal component-artificial neural network model methods for their simultaneous determination [J] . N. Zeinali, M. Ghaedi, G. Shafie Journal of industrial and engineering chemistry . 2014,第5期

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4. The combination of the electrophoretic and electronic microscopy methods for determination of localization of the thallium-containing synthetic antioxidants on the surface of the plague microbe cells [C] . Saratov meeting : Optical technologies in biophysics and medicine . 2002

机译：电泳和电子显微镜方法的组合测定含铊合成抗氧化剂的定位在瘟疫微生物细胞表面上的定位
5. Novel methods for the identification and counting of functional groups in oxygen-containing compounds and sequencing of peptides by ion -molecule reactions in a FT-ICR mass spectrometer. [D] . Somuramasami, Jayalakshmi. 2006

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6. Rapid Catalytic Reduction of 4-Nitrophenol and Clock Reaction of Methylene Blue using Copper Nanowires [O] . Aina Shasha Hashimi, Muhammad Amirul Nazhif Mohd Nohan, Siew Xian Chin, 2019

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7. The bacterial identification and antimicrobial susceptibility testing are usually performed manually. Recently, the number of samples has increased and the need for automatification has grown. BioMerieux has investigated Vitek2 for this purpose. HUSLAB has assigned us to compare the identification and antimicrobial results with respect to routine methods and Vitek2. Furthermore, we evaluate test reactions, ESBL (Extended Spectrum Beta-Lactamase) production and the time what it takes to get results with Vitek2. In this study, we examined a total of 183 bacterial isolates including 76 ESBL-strains, 40 nonfermenting rods, 41 fastidious bacteria and 26 fresh clinical isolates. The results of routine methods were gathered from the patient database and following this, compared to the results given by Vitek2. Vitek2 correctly identified more than 96 f the ESBL-strains and fresh clinical isolates. The results of nonfermenting rods and fastidious bacteri a were 70 oncordant with those of the routine methods. Test reaction results of Api20E were 94 nd Api20NE 70 n concordance with Vitek2 results. Approximately 80 f all Vitek2 antimicrobial results and 90 f ESBL test results were correct. Compared to earlier studies, Vitek2 performed the tests faster than the routine methods. These results suggest that Vitek2 is capable of giving rapid and accurate results. However, nonfermenting rods and fastidious bacteria should be studied further. [O] . Järveläinen Marika, Roslund Iira, Silander Maarit 2006

机译：细菌鉴定和抗菌药敏试验通常是手动进行的。最近，样品的数量增加了，并且对自动化的需求也在增长。 BioMerieux为此进行了Vitek2的研究。 HUSLAB已指派我们比较常规方法和Vitek2的鉴定和抗菌结果。此外，我们还评估了测试反应，ESBL（扩展频谱β-内酰胺酶）的产生以及使用Vitek2获得结果所需的时间。在这项研究中，我们检查了总共183个细菌分离株，包括76个ESBL菌株，40个非发酵棒，41个营养细菌和26个新鲜临床分离株。从患者数据库中收集常规方法的结果，然后将其与Vitek2给出的结果进行比较。 Vitek2正确鉴定出超过96种ESBL菌株和新鲜的临床分离株。与常规方法相比，非发酵棒和精制细菌的结果为70。 Api20E的测试反应结果与Vitek2结果一致为94nd Api20NE 70 n。所有Vitek2抗菌结果约80 f和ESBL测试结果90 f正确。与早期的研究相比，Vitek2的测试速度快于常规方法。这些结果表明Vitek2能够给出快速而准确的结果。但是，应进一步研究非发酵棒和营养细菌。

The Use of the Method of Reduction of Methylene Blue for the Rapid Determination of the Living Bacteria Count when Growing the Plague Microbe by the Depth Method in the Reaction Tank

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