A mathematical model for the radial flow chromatography (RFC) was established, and the effects of volume flow rate (F), height of the column (h) on the elution profile and column efficiency were discussed.Based on the direction of the study of the established model, and using a RFC with diameter of 5 em, the experimental study of deproteinization of Ganoderma lucidum polysaccharides was carried out. The results show that the column efficiency can be stable and efficient when the F/h ratio reaches 10 mL·min-1/cm, and when the F/h ratio is constant, the column efficiency can keep invariant. Thus, the volume flow rate can be linearly enlarged with the increase of the height of RFC. The maximum error between the model calculation and experiment is below 8.8％. According to the guide of the model and based on the experimental equipment used (h = 5 cm), the volume flow rate used during the deproteinization experiment is 50 mL.min-1. The results show that, using the radial flow column, the deproteinization ratio and polysaccharides recovery ratio is 38.2％ and 30.5％, respectively, which are higher than those of using chemical method. The above results provide theoretical basis for the linear amplification characteristic of the RFC as well as for the production of Ganodermna lucidum polysaccharides with RFC.%建立了径向流色谱数学模型,应用MATLAB仿真软件讨论了体积流速和色谱柱高对流出曲线和柱效的影响;应用数学模型指导试验操作参数选择,将径向流色谱应用于灵芝多糖脱除蛋白质的研究.研究结果表明:体积流速F(mL·min-1)和色谱柱高h(cm)的比值(F/h)需要达到10时柱效才会稳定并且高效;且当F/h为定值时,柱效保持不变,因此,径向流色谱体积流速可随柱高进行线性放大.通过验证试验,模型值与试验值最大误差低于8.8%.根据模型指导及试验设备,确定在F/h=10的情况下,径向流色谱脱除灵芝多糖蛋白质试验的体积流速为50 mL.min-1径向流色谱蛋白脱除率及多糖回收率比传统化学方法分别高出38.2%和30.5%.论文工作为径向流色谱的线性放大提供了理论依据,并为灵芝多糖的规模化生产提供了参考.
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