目的 探讨肺癌中谷胱甘肽S-转移酶P1(GSTP1)表达水平及临床病理学意义.方法 应用免疫组织化学方法检测50例原发性肺腺癌、20例癌旁正常组织、11例肺转移性腺癌和14例肺鳞癌组织石蜡标本GSTP1蛋白表达水平,观察其与肺癌患者临床病理学参数的关系.采用Western blot检测8例新鲜肺腺癌、鳞癌及癌旁组织中GSTP1蛋白表达水平.结果 GSTP1在32例原发性肺腺癌及9例肺鳞癌组织中呈高表达,明显高于癌旁正常组织表达水平(x2=6.665,P=0.010; x2=3.927,P=0.048),但在原发性肺腺癌、肺鳞癌及肺转移性腺癌中表达无明显差异(P＞0.05).GSTP1表达与肺腺癌患者年龄、性别、肿瘤大小、分化程度及淋巴结转移无明显相关性(P＞0.05),但与肺腺癌患者预后呈负相关(x2=4.171,P=0.041).Western blot证实GSTP1在肺腺癌及鳞癌中表达明显高于癌旁组织(t =2.545,P=0.023；t =2.337,P=0.035).结论 GSTP1在肺癌中表达上调,并与肺腺癌患者预后相关.%Objective To study the clinicopathological significance of the expression of GSTP1 protein in lung cancer. Methods The expression of GSTP1 protein was detected by immunohistochemistry in 50 cases of primary lung adenocarcinoma specimens, 20 adjacent non-cancerous lung tissues, 11 metastatic pulmonary adenocarcinoma and 14 squamous cell carcinoma tissues. Western blot was used to examine the GSTP1 protein level in 8 cases of lung adenocarcinoma, squamous cell carcinoma and adjuvant non-cancerous tissues. The relationship between the protein expression and clinicopathological features was analyzed. Results Overexpression of GSTP1 protein was shown in 32 and 9 cases of lung adenocarcinoma and squamous cell carcinoma tissues, respectively, which were much higher than that in adjuvant non-cancerous lung tissues (χ2=6.655,P =0.010;χ2=3.927,P =0.048),but there was no statistically significant difference of GSTP1 expression between primary lung adenocarcinoma, metastatic pulmonary adenocarcinoma and squamous cell carcinoma tissues (P > 0.05). The expression of GSTP1 protein was not correlated with age, sex, tumor size, differentiation or lymph node metastasis (P > 0.05), but had a negative association with the prognosis of the lung adenocarcinoma patients (χ2=4.171 ,P =0.041). The expression of GSTP1 protein was higher in lung adenocarcinoma and squamous cell carcinoma tissues than that in non-cancerous lung tissues detected by Western blot (t =2.545, P = 0.023 ;t =2.337 ,P =0.035). Conclusion GSTP1 is up-regulated in lung cancer and positively correlated with the prognosis of the lung adenocarcinoma patients.
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