Objective To explore the role of A-kinase anchoring proteins (AKAPs) in meiotic arrest of mouse oocytes. Methods Ht31 or Ht31-P peptide was microinjected into GV-stage mouse oocytes that were incubated in the presence of dbcAMP. The GVBD rates were counted and the phosphorylation status of Cdc2-Tyrl5 was determined by western blot. Results Ht31 stimulated oocyte maturation as indicated by GVBD and dephosphorylation of Cdc2-Tyrl5,which overcame the dbcAMP-induced arrest. In the control group,the inactive Ht31-P had no effect on GVBD,and Cdc2-Tyrl5 was phosphorylated. Conclusion PKA (protein kinase A) interaction with AKAPs is essential for the initial maintenance of meiotic arrest in mouse oocytes.%目的 探讨A型激酶锚定蛋白(AKAPs)在小鼠卵母细胞减数分裂阻滞中的作用.方法 收集生发泡期小鼠卵母细胞,显微注射Ht31或Ht31-P,观察卵母细胞减数分裂恢复情况.结果 在蛋白激酶A(PKA)持续激活的情况下,Ht31注射组能促进小鼠卵母细胞减数分裂重新启动,解除PKA引起的生发泡期阻滞,而Ht31-P对照组不能.结论 AKAPs介导的PKA正确锚定对小鼠卵母细胞第一次减数分裂阻滞至关重要.
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