建立了超高效液相色谱-三重四极杆-线性离子阱质谱(UPLC-Qtrap-MS/MS)同时测定玄参药材中环烯醚萜苷类、苯丙素苷类和有机酸类等12种功效成分的方法,分析了不同加工方法对玄参药材中多元功效成分含量的影响,并采用UPLC-Qtrap-MS/MS技术同时测定其中的12种功效成分含量,用灰色关联度分析法对多元功效成分进行综合评价.结果表明,12种功效成分在一定浓度范围内的线性关系良好,相关系数均大于0.999 0,加标回收率为95.82%~99.35%,相对标准偏差(RSD)小于2.44%;不同加工方法对玄参中多元功效成分含量具有一定的影响,灰色关联度分析结果显示,阴干法和完整药材蒸后烘干的样品综合质量较好.该实验揭示了加工对玄参中多元功效成分的影响,可为优选玄参适宜产地加工方法提供基础资料,同时为玄参药材内在质量的综合评价提供方法参考.%Scrophulariae Radix is one of the most popular traditional Chinese medicines (TCMs).Primary processing of Scrophulariae Radix is an important link which closely related to the quality of products in this TCM.A method of ultra high performance coupled with triple quadrupole liner ion trap mass spectrometry (UPLC-Qtrap-MS/MS) was established for the determination of iridoid, phenylpropanoid glycosides and organic acids in Scrophulariae Radix.The goal of the method was to investigate the influences on multiple active constituents with different processing methods of Scrophulariae Radix. Twelve multiple active constituents in Scrophulariae Radix with different processing methods were simultaneous determined by UPLC-Qtrap-MS/MS, and grey incidence degree method analysis was performed to evaluate the different processed samples according to the contents of twelve multiple active constituents.The analysis was carried out on an BDS HYPERSIL C18 column (250 mm×4.6 mm×5 μm), with elution of acetonitrile-water as mobile phase at a flow rate of 1.0 mL/min.The column temperature was 35 ℃.The target compounds were analyzed by the negative ion multiple reaction monitoring (MRM) mode.The results show that twelve multiple active constituents have good linearity (r≥0.999 0) in the range of the tested concentration.The average recoveries of the twelve components are 95.82%-99.35% with the relative standard deviations less than 2.44%.There are differences in multiple active constituents of Scrophulariae Radix with different processing methods and the processing method of air drying method and complete root by oven drying after steaming had a better quality.The method is useful for the overall assessment on quality of Scrophulariae Radix, and this study may provide the foundation and support for processing method of Scrophulariae Radix in normalization and standardization.
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