目的：探讨与分析氨基胍对糖尿病大鼠肾脏非酶糖基化的抑制作用。方法选取90只大鼠作为研究对象，采取随机数字表法分为 A、B、C 三组。A、B 两组均喂养高糖高脂肪饮食及皮下注射小剂量链脲佐菌素建立2型糖尿病大鼠模型，A 组大鼠定期服用含有氨基胍的饮用水，B 组大鼠定期服用等量不含氨基胍的正常饮用水，C 组大鼠定期服用等量不含氨基胍的正常饮用水，设定为对照组。对比三组大鼠治疗后体内高级糖基化产物的变化情况，血肌酐、血尿素氮、内生肌酐清除率、尿蛋白排泄量水平以及肾脏基底膜厚度、肾脏系膜体积变化情况。结果治疗前后，A 组大鼠治疗后高级糖基化产物明显高于治疗前，B 组治疗后明显高于治疗前，C 组大鼠治疗前后无明显变化，但 A 组治疗后糖基化产物的含量明显低于 B 组，组间相比具有显著差异（ P ＜0.05）。治疗后 A 组和 B 组大鼠体内的血肌酐水平、血尿素氮水平、内生肌酐清除率、尿蛋白排泄量均明显升高，C 组无明显变化，但 A 组大鼠的各项指标明显低于 B 组，组间相比具有显著差异（ P ＜0.05）。治疗5周、10周、15周后，A 组和 B 组大鼠肾脏的基底膜厚度和系膜体积均明显增加，C 组无明显变化，但 A 组大鼠肾脏的基底膜厚度和系膜体积明显小于 B 组，组间相比具有显著差异（ P ＜0.05）。结论氨基胍可以通过抑制高级糖基化产物的生成，从而减少其在大鼠肾脏的蓄积，并显著降低尿蛋白的排泄、抑制系膜的增生，在一定程度上防止糖尿病向糖尿病肾病的发展，并且氨基胍的抑制作用与使用时间呈正相关。%Objective To explore and analyze the effect of aminoguanidine on inhibition of non - enzymatic glycation in kidney of diabetic rats. Methods A total of 90 rats were allocated as study subjects,they were randomly divided into A,B and C three groups,rats in groups A and B were fed with high fat and sugar diet and subcutaneously injection of low - dosage of streptozotocin in order to establish type 2 diabetic rat mod-els,rats in group A were administered with drinking water containing regular aminoguanidine,and rats in group B were drinking in same amount of water without aminoguanidine,and rats in group C were not regularly taken the same amount of aminoguanidine in normal drinking water and set as control group. After comparing the changes in three groups of rats treated with advanced glycosylation products,and changes in levels of serum creatinine,blood urea nitrogen,creatinine clearance,urinary protein excretion and thickness of basement membrane and changes in renal mesang-ial volume were compared among these 3 groups. Results After treatment,the advanced glycosylated products in rats of group A and group B were significantly higher than those before treatment,and this product in rats of group C had no significant change before and after treatment,but content of glycosylated product in rats of group A after treatment was significantly lower than that of rats in group B,and the difference was signifi-cant( P < 0. 05)between these two groups. After treatment,blood levels of creatinine and urea nitrogen,creatinine clearance and urinary protein excretion in rats of group A and group B were significantly increased,rats in group C had no significant change,but these indicators in rats of group A were significantly lower than those of rats in group B,and the difference was significant( P < 0. 05)between these two groups. After treatment for five weeks,10 weeks and 15 weeks,the thickness of basement membrane and volume of renal mesangium in rats of group A and group B were significantly increased,and the change in rats of group C was not significant,but the thickness of basement membrane and volume of renal mesan-gium in rats of group A were significantly less than those of rats in group B,and the difference was significant( P < 0. 05). Conclusion Amin-oguanidine can inhibit the formation of advanced glycation products,thereby reducing their accumulation in kidney of rats and the decreased urina-ry protein excretion can significantly inhibit the proliferation of mesangium to a certain extent,hence it may prevent the development of diabetes and diabetic nephropathy,hence the inhibition of aminoguanidine is positively correlated with the duration for utilization.
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