目的 探讨睾酮对慢性心力衰竭雄性大鼠心脏纤维化的影响. 方法 将53只雄性SD大鼠分为正常对照组(A组)、睾丸切除加心力衰竭组(B组)、睾丸切除加心力衰竭加睾酮(T)替代治疗组(C组).应用放射免疫法检测大鼠血浆T和肿瘤坏死因子-α(TNF-α)水平,免疫组化检测心肌组织转化生长因子-β1(TGF-β1)表达水平,VG染色观察大鼠心肌间质胶原纤维的变化.结果 与B组比较,C组TNF -α水平明显降低[C组vs.B组:(0.86±0.27)ng/mL Vs(1.28±0.26) ng/mL,P＜0.01],TGF -B1水平明显降低[C组vs.B组:(3.32±1.61)％vs(6.49±2.82)％,P＜0.01],胶原容积分数( CVF)减少[C组vs.B组:(2.13±0.72)％vs(2.84±0.79)％,P＜0.05].结论 睾酮能抑制心力衰竭雄性大鼠心肌纤维化,从而保护心功能,其作用的一个重要机制可能为睾酮拮抗TGF -β1的产生.%Objective To investigate the effect of testosterone on myocardial fibrosis of male rats with chronic heart failure (HF). Methods Fifty - three male SD rats were divided into 3 groups; control group ( group A), orchiectomy + HF ( group B), and orchiectomy + HF + testosterone ( T ) (group C). The levels of testosterone and cytokine(TNF -α) were measured by radioimmunoassay, TGF - p, was detected by immunohistochemical method, and interstitial collagen of myocardial cell was observed by V - G staining. Results Compared with group B, plasma TNF - a level was dramatically decreased in group C (group C 0. 86 ±0. 27 ng/mL and group B 1. 28 ±0. 26 ng/mL, P <0. 01) , plasma TGF -β1, level was significantly reduced (group C 3. 32 ± 1. 61% and group B 6. 49 ± 2. 82% , P <0.01) , and the collagen volume fraction dropped as well (group C 2. 13 ± 0. 72% and group B 2.84 ± 0.79% , P < 0.05). Conclusion In male rats with HF, testosterone exerts a suppres-sive effect on myocardial fibrosis mostly due to its inhibition to TGF - β1.
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