Objective To investigate the changes of major histocompatibility complex class II (MHC- II) and cluster of differentiation 3ε (CD3ε) mRNA in brain in mouse with experimental autoimmune encephalomyelitis ( EAE). Methods Twenty-five C57BL/6 mice were divided randomly into EAE group { n = 13 ) and normal control group (n — 12). EAE group was induced into EAE models by myelin oligodendrocyte glycoproleiit 35-55 peptide. The behavioral changes of the mice were observed. The degrees of injury and inflammatory cells infiltrating in spinal cord were observe by HE and meylin staining; the expression of MHC-II and CD3e mRNA in brain were assayed by fluorescent quantitation PCR. Results EAE symptom scores in EAE group mice were gradually increased alter EAE onset,the inflammatory cells infiltrating in spinal cord was obvious,the myelin accounting·was more,the expression of MHC-II and CD3ε in brain were up-regulated than normal control group (all P <0. 01) , and which were positively correlated with EAE symptom scores. Conclusion The increase expression of MHC- II and CD3ε mRNA in brain in EAE mouse were correlation with the EAE severity.%目的 观察实验性白身免疫性脑脊髓炎(EAE)小鼠脑内主要组织相容性复合体Ⅱ类抗原(MHC-Ⅱ)和分化群3ε(CD3ε)的变化.方法 25只C57 BL/6小鼠随机分为EAE组(n=13)和正常对照组(n＝12).应用髓鞘少突胶质细胞糖蛋白35-55抗原诱导小鼠EAE模型.观察记录小鼠行为学变化；采用常规及髓鞘染色方法观察脊髓损伤和炎症细胞浸润程度；荧光定量PCR检测脑MHC-Ⅱ和CD3ε mRNA的表达.结果 EAE组小鼠发病后EAE症状评分逐渐增加；脊髓炎症细胞浸润明显；髓鞘脱失较多；脑组织MHC-Ⅱ和CD3εmRNA表达显著高于正常对照组(均P＜0.01),并与EAE症状评分呈正相关.结论 EAE小鼠脑内MHC-Ⅱ及CD3ε mRNA表达水平增高与其病情严重程度一致.
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