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首页> 中文期刊> 《临床儿科杂志》 >地塞米松对脓毒症新生大鼠nephrin的影响

地塞米松对脓毒症新生大鼠nephrin的影响

         
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摘要

Objective To observe the effects of dexamethasone on the expression of nephrin and P-cadherin at the slit diaphragm of the glomerular podocytes in neonate rats with sepsis, and investigate the mechanism of proteinuria in sepsis. Methods Twenty-four 10-day-old Wistar rats were intraperitoneally injected with a single dose of lipopolysac-charide (LPS) to induce sepsis model, and randomly divided into sepsis group (SE, n = 12) and SE plus dexamethasone treatment group (DE, n = 12). Rats in DE group were administrated dexamethasone (0.3 mg/kg) by intraperi-toneal injection at 12 h after inrraperitoneal injection of LPS. Another 10-day-old Wistar rats were served as control group (NC, n = 10). At 48 h after LPS was intraperitoneally injected, serum C-reactive protein (CRP), procalcitonin (PCT), neutrophil gelatinase-associated lipocalin (NGAL), 24 h urinary protein (24 h UP) and urinary podocytes (UPC) were measured. The expressions of glomerular nephrin and P-cadherin mRNA and proteins were detected by RT-PCR and Western-blotting respectively. Results CRP, PCT, NGAL and 24 h UP were significantly higher in SE group than those in NC group (P < 0.01) , and UPC had no significant difference. The nephrin and P-cadherin mRNA and proteins expressions were decreased in SE group than those in NC group. Dexamethasone significantly reduced CRP, PCT, NGAL, 24 h UP levels which were induced by LPS challenge, and preserved the expressions of nephrin and P-cadherin mRNA and proteins, but had no effect on the excretion of UPC. Conclusions Dexamethasone can ameliorate systemic inflammatory response, restore the expressions of glomerular nephrin and P-cadherin mRNA and proteins, and reduce proteinuria.%目的 观察脓毒症新生鼠肾小球足细胞裂孔隔膜蛋白nephrin、P-cadherin的表达及地塞米松对两者的影响,以探讨脓毒症蛋白尿的发生机制.方法 新生10 d Wistar大鼠24只,采用脂多糖(LPS)腹腔内注射的方法诱导脓毒症模型,随机分为脓毒症组(SE组,n=12),地塞米松治疗组(DE组,n=12),DE组大鼠腹腔注射LPS 12h后给予地塞米松0.3 mg/kg.另设出生10 d Wistar鼠为对照组(NC,n=10).注射LPS48 h后分别测定血清C-反应蛋白(CRP)、降钙素原(PCT)、中性粒细胞明胶酶相关载脂蛋白(NGAL),24h尿蛋白(24 hUP)及尿足细胞(UPC)排泄水平；RT-PCR和Western-blotting检测肾小球nephrin、P-cadherin mRNA和蛋白质的表达.结果 与NC组相比,SE组大鼠的CRP、PCT、NGAL、24hUP水平均显著升高,差异有统计学意义(P均＜ 0.01),UPC差异无统计学意义；nephrin、P-cadherin mRNA及蛋白质的表达明显下调.地塞米松明显改善LPS引起的CRP、PCT、NGAL、24h UP升高,维持nephrin、P-cadherin mRNA及蛋白质的表达,对UPC的排泄无明显影响.结论 地塞米松可减轻全身炎症反应,恢复肾小球nephrin、P-cadherin mRNA和蛋白质的表达,减轻蛋白尿.

著录项

  • 来源
    《临床儿科杂志》 |2012年第4期|367-371|共5页
  • 作者

    邹敏书; 周建华; 余健; 何威逊; 聂国明; 罗莉漫; 徐洪涛; 丁冬胜; 李琳;

  • 作者单位

    广州军区武汉总医院儿科,湖北武汉430070;

    华中科技大学同济医学院同济医院儿科,湖北武汉430030;

    华中科技大学同济医学院同济医院儿科,湖北武汉430030;

    广州军区武汉总医院儿科,湖北武汉430070;

    上海交通大学附属上海儿童医院肾脏科,上海200040;

    广州军区武汉总医院儿科,湖北武汉430070;

    广州军区武汉总医院儿科,湖北武汉430070;

    广州军区武汉总医院儿科,湖北武汉430070;

    广州军区武汉总医院儿科,湖北武汉430070;

    广州军区武汉总医院儿科,湖北武汉430070;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 动物学的研究与实验;
  • 关键词

    地塞米松; 脓毒症; nephrin; 蛋白尿;

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