Objective: To explore the effect of RSVA314 on the migration and proliferation of leukemic K562 cells.Methods: The leukemic K562 cells were cultured in vitro and four groups were set up: control group, low dose of RSVA314 (1μmol/L) group;middle dose of RSV314 (5 μmol/L) group and high dose of RSVA314 (25μmol/L) group.The proliferation activity of leukemic K562 cells was detected by measuring the OD value with the spectrophotometer.Transwell migration assay was used to detect the migration ability of K562 cells.The expression of p-AKT in K562 cells was detected by Western blotting.Results: The OD value was increased significantly in the middle and high dose of RSVA314 groups as compared with the control group (P<0.05, or P<0.01), indicating that RSV314 could inhibit the proliferation of K562 cells.As compared with the control group, the migration ability in RSVA314-treated groups was inhibited (P<0.05 or P<0.01).The results of Western blotting showed that RSVA314 could inhibit the expression of p-AKT in the middle and high dose of RSVA314 groups, and there was significant difference as compared with the control group (P<0.05 or P<0.01).Conclusion: RSVA314 can inhibit the migration and proliferation of leukemic K562 cells partly by inhibiting the expression of p-AKT.%目的:探讨白藜芦醇的小分子类似物(RSVA314)对白血病K562 细胞迁移和增殖的影响.方法:体外培养K562 细胞,并分为4组:生理盐水对照组、RSVA314低剂量组(1 μM)、RSVA314中剂量组(5 μM)、RSVA314高剂量组(25 μM).采用CCK-8方法分别检测4组中K562细胞的增殖活性;采用TransweⅡ 迁移实验分别检测4组K562细胞的迁移能力;采用Western blot分别检测4组K562细胞蛋白激酶B(AKT)的表达.结果:与对照组比较,RSVA314中、高剂量组OD值明显增加(P<0.05或P<0.01),提示其可抑制K562细胞的增殖活性.RSVA314低、中、高剂量组穿膜细胞数量明显减少,与对照组比较有明显差异(P<0.05或P<0.01).Western blot 检测显示RSVA314中、高浓度组可抑制AKT磷酸化的表达,与对照组比较有显著差异(P<0.05或P<0.01).结论:RSVA314能抑制白血病K562 细胞迁移和增殖,其机制可能与抑制K562细胞AKT磷酸化表达有关.
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