Toxic effect of suspension and lixivium from the dredged sludge of the Yangshan channel project on megalopa of Portunus trituberculatus was evaluated, through a laboratory simulation experiment. Results show that ( 1) mortality of the megalopa increased gradually with concentration of the suspension and lixivium, and 96 h LC50 and SC of the lixivium to megalopa of Portunus trituberculatus was 1 294�66 and 129�466 mL·L-1, respectively, and of the suspension, 996�17 and 99�617 mL·L-1 , respectively;( 2) molting rate of the megalopa decreased with increasing concentration of the sus⁃pension and lixivium;it was slightly higher in the group of lixivium treatments than in the group of suspension treatments group when they were the same in volume concentration;the suspension inhibited molting of megalopa, while the lixivium inhibited hardening of new shell;( 3) After 24 hours of exposure to the suspension and lixivium, the expression of HSP70 in the muscle of megalopa increased rapidly; after 72 hours, it peaked in all the treatments; and after 96 hours, it de⁃creased to a varying extent in all the treatments and the most significantly in the treatment the highest in concentration;while in the control it kept on rising.%研究了洋山航道工程疏浚泥悬浮液和浸出液对三疣梭子蟹（ Portunus trituberculatus）大眼幼体的毒害效应，结果表明：（1）大眼幼体死亡率随着悬浮液和浸出液配比浓度的增大而逐渐增加。浸出液对大眼幼体的96 h半致死配比浓度φ（ LC50）为1294�66 mL·L－1，安全配比浓度φ（ SC）为129�466 mL·L－1；悬浮液对大眼幼体的96 hφ（LC50）为996�17 mL·L－1，φ（SC）为99�617 mL·L－1。（2）大眼幼体的蜕皮率随着悬浮液和浸出液配比浓度的增加而逐渐降低。同体积配比浓度下，浸出液处理组蜕皮率略高于悬浮液处理组。悬浮液抑制三疣梭子蟹大眼幼体的蜕壳，而浸出液抑制新壳的硬化。（ 3）浸出液和悬浮液胁迫24 h后，大眼幼体肌肉中HSP70表达量迅速升高，浸出液和悬浮液最高配比浓度组表达量显著高于对照组（ P＜0�05）；72 h各处理组HSP70表达量均达到最高值；96 h后各处理组表达量有所降低，最高配比浓度组HSP70表达量降幅明显（ P＜0�05），对照组表达量仍继续上升。
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