Objective:To explore the prevention effect of FZHY recipe on the formation of acute on chronic liver failure.Methods:66 male Wistar rats were randomly divided into normal control group (6),acute liver failure group (8),chronic.hepatitis model group (6),chronic hepatitis intervention group (6),acute on chronic liver failure model group(20),cute on chronic liver failure intervention group(20).50％ carbon tetrachloride vegetable oil solution was intraperitoneally injected to establish liver injury models,FZHY recipe was dosed to intervention group.After 9 weeks,D-galactosamine was simultaneously intraperitoneally injected to normal rats of chronic hepatitis,chronic hepatitis model rats and the intervention group to establish acute liver failure or acute on chronic liver failure models.In 12 hours after the acute attack,the mortality,liver function,liver pathological changes were observed.Results:In the ninth week,chronic hepatitis model group had higher liver/body rate and worse liver function than the nomal group and pathologically liver cell injury,inflammatory cells invasion were detected in the chronic hepatitis model group.Compared with chronic hepatitis model group,liver/body rate and liver function in chronic hepatitis intervention group had been improved obviously,including liver cell injury and inflammatory cells invasion.In 12 hours after the acute injuries,the mortality rates in acute liver failure group,chronic on acute hepatic failure model group and chronic on acute hepatic failure intervention group were 0％,100％ and 25％ respectively.Acute liver failure group and acute on chronic hepatic failure model group had significantly worse liver function,pathologically massive necrosis of liver cells and infiltration of inflammatory cells than the normal group.Compared with chronic on acute hepatic failure model group,liver function had been improved significantly in chronic on acute hepatic failure intervention group,including necrosis of liver cells and infiltration of inflammatory cells pathologically.Conclusion:FZHY recipe can reduce severity and mortality of acute on chronic liver failure significantly,has significant prevention effect in the formation of acute on chronic liver failure in rats suffering from chronic liver injury.%目的 观察扶正化瘀方干预治疗对于慢加急性肝衰竭(ACLF)形成及严重程度的影响.方法 66只雄性Wistar大鼠随机分成正常对照组(6只)、急性肝衰竭组(8只)、慢性肝炎模型组(6只)、慢性肝炎干预组(6只),ACLF模型组(20只)、ACLF干预组(20只),采用50％ CCL植物油溶液腹腔注射建立慢性肝炎模型,干预组予扶正化瘀方灌胃干预治疗,第9周用D-氨基半乳糖急性攻击正常大鼠、慢性肝炎模型大鼠和慢性肝炎干预组大鼠建立急性肝衰竭或ACLF模型,12 h后观察各组死亡率、肝功能及肝脏病理等变化.结果 大鼠造模9周,慢性肝炎模型组大鼠较正常组大鼠肝体比显著升高,肝功能指标明显恶化;病理显示肝细胞损伤,炎细胞侵润.慢性肝炎干预组肝体比和肝功能指标较慢性肝炎模型组有明显改善;病理上炎症明显减轻.急性损伤后12h,急性肝衰竭组、ACLF模型组、ACLF干预组死亡率分别为0％、100.00％、25.00％.急性肝衰竭组、ACLF模型组肝功能指标较正常组明显恶化,病理见大量肝细胞坏死和炎细胞浸润.而ACLF干预组较ACLF模型组肝功能指标和肝细胞坏死和炎细胞浸润明显减轻.结论 扶正化瘀方明显减轻ACLF形成时的严重程度,降低死亡率,对于ACLF有着积极的预防作用.
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