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胭脂鱼听觉阈值研究

         

摘要

利用听性脑干反应技术研究了胭脂鱼(Myxocyprinus asiaticus)的听觉阈值。通过插入皮下的电极记录了10尾胭脂鱼的短纯音听觉诱发电位。结果显示,在系统最大声强下,胭脂鱼的听频范围为100~5000 Hz,其中对100~2000 Hz的声音敏感度较高,最敏感的频率为800 Hz,听觉阈值约为69.8 dB。随着声强的减小,胭脂鱼的听性脑干反应波形最大,振幅减小,潜伏期延长。将10尾胭脂鱼的阈值平均,得到了胭脂鱼的听性脑干反应听力图。胭脂鱼的听力图呈“U”形,属于典型的动物听力曲线。研究胭脂鱼的听觉阈值对胭脂鱼物种保护具有重要意义,同时可为评估噪声对胭脂鱼的影响提供数据支持。%Auditory brainstem response (ABR) technic has been widely used to study hearing ability in fishes. In contrast to the behavior and traditional electrophysiology methods, this approach provides the following advan-tages:(1) there is no harm to the animals because it is non-invasive;(2) hearing sensitivity in untrained fish can be determined rapidly; (3) individuals are reusable. Using ABR approach, auditory evoked potentials of 10 Chinese suckers Myxocyprinus asiaticus were recorded using two subcutaneous electrodes. We found that the fish can de-tected tone bursts from 100 to 5 000 Hz. The results showed the best hearing between 100 to 2 000 Hz, and the lowest threshold was 69.8 dB at 800 Hz. There was a positive correlation between the amplitude of the ABR waveform and the stimulus intensity, while there was a negative correlation between latency and stimulus intensity. Threshold values from 10 individuals were averaged to produce audiogram. Similarly to the most audiograms, it is a U-shape with thresholds increasing at both high and low frequency limits of hearing. The results have an impor-tant significance in protecting Chinese sucker and providing supporting data for access the effect of noise on fish-es.

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