目的 建立基于简并寡核苷酸引物PCR(degenerate oligonucleotide primed-PCR,DOP-PCR)的全基因组检测体系,并对其可靠性、灵敏度进行研究.方法 通过荧光标记STR复合扩增毛细管电泳检测系统,对DOP-PCR扩增产物进行检测,获得DOP-PCR检测体系的灵敏度、可靠性. 结果 成功建立了可靠的DOP-PCR检测体系,经过DOP-PCR预处理再进行STR分型检验,其检测灵敏度可达到5个细胞量(相当于30pg).结论 本研究建立的DOP-PCR技术可靠且可提高法医学微量检材的检测灵敏度.%Objective To establish a whole genome amplification testing system based on degenerate oligonu-cleotide primed-PCR(DOP-PCR) and to explore its reliability and sensitivity. Methods DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophore-sis detection system to determine reliability and sensitivity of DOP-PCR system. Results DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30pg) by pretreatment of DOP-PCR and then detection of STR genotyping. Conclusion The system established in this study is reliable and more testing sensitive for forensic trace evidence.
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