目的 探讨增强化学发光法检测人基质金属蛋白酶-11(matrix metalloproteinase-11,MMP- 11)鉴定月经血的法医学应用价值.方法 运用增强化学发光法检测月经血痕、阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中的MMP-11,考察MMP-11的组织特异性、稳定性及检测方法的灵敏度.结果 月经血痕中MMP-11蛋白的检测阳性率为89.47％,阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中均未检出MMP-11.当上样量为25 μL时,蛋白质量浓度为1.329 μg/μL,可检出MMP-11.4℃放置20个月的MMP-11阳性的月经血痕,检测阳性率为89.58％.结论 用增强化学发光法检测MMP- 11蛋白的方法灵敏度高、特异性好,能用于月经血与外周血、阴道液等常见斑痕的鉴别.%Objective To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method. Methods Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated. Results The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 μL sample was added, the mass concentration of protein was 1.329 μg/μL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4℃ for 20 months. Conclusion Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.
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