Objective To investigate the effect and expression of the vascular cell adhesion molecule-1 (VCAM-1) in organs of rats died of anaphylactic shock. Methods The models of anaphylactic shock in rats were made and the immunohistochemisty of SABC was used to detect as follows: (1) The expression of VCAM-1 in rat lung, heart, brain, kidney, liver, spleen, stomach and intestine. (2) VCAM-1 levels in lungs at 10min, 30min after the allergic shock, and the time of death. (3) VCAM-1 levels in lungs of rats after the intervention of anti-VCAM-1. Results After the death, the expression VCAM-1 in lungs increased significantly relative to the control group and followed the extension of shock. In the rats which were injected with the anti-VCAM-l, the expression of VCAM-1 in lungs reduced. Conclusion (1) The expression of VCAM-1 shows difference in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs and would increase followed the extension of anaphylactic shock.(2) After the anaphylacfic shock, anti-VCAM-1 can inhibit the expression of VCAM-1 in rat lung.%目的 探讨血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)在过敏性休克大鼠器官中的作用及其表达规律.方法 制造大鼠过敏性休克模型,采用免疫组化SABC法分别检测:(1)过敏性休克大鼠肺、心、脑、肾、肝、脾、胃、肠中VCAM-1的表达;(2)过敏性休克不同时间(休克10、30min和死亡时)大鼠肺内VCAM-1的表达;(3)在诱导大鼠过敏性休克后用抗VCAM-1干预时大鼠肺内VCAM-1的表达.结果 在过敏性休克大鼠死亡时,肺内VCAM-1的表达与对照组相比显著增多,而且随着休克时间延长,VCAM-1表达有增多趋势,休克后抗VCAM-1处理组肺内的VCAM-1表达相对减少.结论 (1)VCAM-1在过敏性休克大鼠各器官中的表达呈现显著差异性,肺内变化最明显,随着过敏性休克的进展有上升趋势.(2)过敏性休克发生后,抗VCAM-1能抑制大鼠肺内VCAM-1的表达.
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