以苏云金芽孢杆菌(Bt)库斯塔克亚种(subsp.kurstaki) 8010为研究材料,通过PCR技术获得Bt8010的外孢囊蛋白基因上下游序列,以含有卡那基因的pDG780质粒为中间载体,通过酶切、连接和PCR,构建含外孢囊蛋白基因上游序列、卡那基因、外孢囊蛋白基因下游序列的基因敲除载体pRN5101UKD,为后续外孢囊蛋白基因敲除工作做准备.%Bacillus thuringiensis subsp. Kurstaki isolate 8010 was selected to obtain the upstream and downstream of exosporium protein gene by PCR technology. The pDG780 vector ( containing the kanamycin resistance gene) was used to construct a recombination plasmid pRN5101UKD which contained the upstream sequence of exosporium protein gene, the kanamycin resistance gene and the downstream sequence of exosporium protein gene by PCR and RE treatments. It was provided for further study on gene knockout.
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