首页> 中文期刊> 《福建农林大学学报(自然科学版)》 >超声波辅助提取菜用黄麻色素及其抗氧化活性的研究

超声波辅助提取菜用黄麻色素及其抗氧化活性的研究

         

摘要

Extraction technology of pigment from jute with ultrasonic assisted method was determined with single-factor and orthogonal experimental design. Antioxidant activity of extracted pigment was evaluated with Fenton method. The factors affecting extraction rate were ordered as ratios of raw material to solvent > ultrasonic frequency > extraction temperature > extraction time. The optimum conditions were 60:40 (V/V) of 0.1% HCl to 95% alcohol, 1:80 of raw material to solvent, intermediate ultrasonic frequency, extraction time 30 min, extraction temperature 60℃. Under these conditions, yield of jute pigment was 25.04%. The pigment had reducing activity and scavenging capacity on 02- and o OH. Scavenging rate on O2- was 70.11% , that on o OH was 83% when the concentration was 0.20 g o L-1.%采用单因素试验分析和正交试验设计,研究超声波辅助提取菜用黄麻色素的工艺条件,用Fenton法测定菜用黄麻色素的抗氧化活性.结果表明:(1)影响色素提取的因素为料液比>超声频率>提取温度>提取时间;(2)提取的最佳条件为使用60∶40(V/V)的0.1%盐酸和95%乙醇作为提取剂,料液比1∶80(g·mL-1),超声频率为中频,提取时间30 min,提取温度为60℃;以此工艺条件提取色素的产率达25.04%;(3)该色素溶液还原力强,具有清除超氧阴离子自由基O2·-和·OH的活性,在色素浓度为0.20 g·L-1时,对超氧阴离子自由基O2·-的清除率为70.11%,对·OH的清除率为83%.

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