ISSR ( inter-simple sequence repeat) was used to analyze the genetic diversity of 11 cultivars of Rhododendron hybridum.Thirteen primers produced 106 polymorphic sites. The result analyzed by software POP-GENE 32 showed that the genetic diversity level orR. hybridum was high, effective alleles was 1.5564, Nei's gene diversity was 0.3207, Shannon's information index was 0.4742, and the percentage of polymorphic sites was 85.48％. Based on the 8 polymorphic sites amplified by 6 ISSR primers, a computerized ISSR fingerprinting was drawn to facilitate the management of 11 cultivars. The result confirmed that 1SSR markers were available for the identification of R. hybridurn cultivars.%采用ISSR标记对11个比利时杜鹃(Rhododendron hybridum)栽培品种(系)的遗传多样性进行研究.采用13条引物共获得多态性位点106个.POP-GENE 32软件分析结果表明,11个品种(系)的有效等位基因数为1.5564个,Nei's基因多样性指数为0.3207,Shannon多样性指数为0.4742,多态性位点百分率为85.48%,总的遗传多样性水平较高.利用6个标记产生的8个多态位点绘制了11个栽培品种(系)计算机化的ISSR指纹图谱,结果表明,ISSR标记可用于区分比利时杜鹃品种.
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