Objective To study the effect of Nicotiflorin (Kaempferol 3-O-β-rutinoside) on prolif-eration ,cell cycle distribution and apoptosis of human breast cancer cells MCF-7 . Methods M TS assay was performed to detect the viability of MCF-7 cells and flow cytometry were used to observe the effect of Nicotiflorin on cell cycle distribution and apoptosis . Western blot was applied to deteted γ-H2AX at the DNA double-strand breaks (DSBs) of chromatin as well as Bcl-2 expression . Result Nicotiflorin inhibi-ted the proliferation of MCF-7 cells in a dose-dependent manner and IC50 is 28 .2 μmol/L . Nicotiflorin arrested MCF-7 cells at G2/M phase and induced apoptosis . Western blot showed that Nicotiflorin in-duced DSBs and dow n-regulated the expression of Bcl-2 in a time-dependent manner . Conclusion Nicoti-florin has the biological activity to inhibit proliferation ,cell cycle of human breast cancer MCF-7 cell as well as to induce cell apoptosis .%目的：探讨Nicotiflorin（Kaempferol 3-O-β-rutinoside ，山奈酚-3-O-芸香糖苷）对人乳腺癌MCF-7细胞的增殖抑制，以及诱导细胞凋亡和对细胞周期的影响。方法采用M T S法检测细胞存活率，计算IC50值；流式细胞仪检测细胞周期及细胞凋亡；Western-blot检测DNA损伤标记蛋白γ-H2AX及凋亡相关蛋白Bcl-2的变化。结果 Nicotiflorin能剂量依赖性地抑制人乳腺癌MCF-7细胞的增殖，IC50值为28．2μmol/L。流式细胞仪检测结果显示，Nicotiflorin使MCF-7细胞周期阻滞于G2/M 期，不同浓度的Nicotiflorin作用48 h后细胞凋亡率均增加。Western-blot检测发现，Nicotiflorin处理后，磷酸化的 H2AX（γ-H2AX）随时间依赖性地增加，提示 Nicoti-florin诱导DNA双链断裂从而使细胞阻滞于G2/M期，同时Nicotiflorin可能通过抑制抗凋亡蛋白Bcl-2的表达诱导细胞凋亡。结论 Nicotiflorin能抑制人乳腺癌MCF-7细胞的增殖并诱导其凋亡。
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