Polymeric nanoparticles were constructed from hyaluronic acid (HA) and histone (His) as a tumor pH-responsitive anticancer drug carrier. As pH decreased from 7. 4 to 5. 5, the particle size increased from 230 to 780 nm, absolute zeta potential increased, and in vitro DOX release increased. DOX-loaded nanoparticles (< 300nm) showed higher cytotoxicity to MCF-7 cells. Cell uptake studies indicate that DOX uptake might be taken place by two ways: endocytosis mediated by HA receptor and uptake of DOX released from DOX-loaded nanoparticles. Consequently, the findings indicate that His-HA nanoparticles are pH-responsive and show a promising application as a doxorubicin carrier.%采用环氧树脂/聚乙二醇嵌段共聚物为乳化剂及相反转法制备了环氧树脂E-44水乳液,探讨了乳化剂用量对相反转点的影响,以及相反转搅拌速度对环氧树脂乳胶粒子粒径的影响.结果表明,随乳化剂用量的增加,相反转点变小；随搅拌速度的增加,乳液的平均粒径显著减小.探讨了丙烯酸酯类混合单体及聚合反应对环氧树脂乳胶粒的溶胀行为.结果表明,混合单体对环氧树脂乳胶粒子有溶胀作用,当单体与环氧树脂的质量比(Mm/MEP)达到0.8时,乳胶粒被胀破,但复合预乳液的稳定性并未下降；聚合后,环氧树脂乳胶粒的平均粒径进一步增大且分布变宽,当Mm/MEP达到0.6后,部分乳胶粒因粒径过大而发生沉降.
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