目的:探讨重组人白介素10 (IL-10)对急性坏死性胰腺炎(ANP)血清IL-6水平的影响,为临床治疗ANP提供新的途径及理论依据.方法:健康雄性SD大鼠92只,随机分成正常对照组(C组)、ANP组(A组)和IL-10干预组(I组).A组大鼠分3次给予腹腔内注射6%的左旋精氨酸(L-Arginine)3×1.0 mg/g体重,每次间隔1 h,诱导ANP;I组于L-Arginine注射诱导胰腺炎后2,5,8 h分别腹腔内注射重组人IL-10 1万U,共3万U;C组大鼠按上述时点给予0.9%生理盐水腹腔内注射作正常对照.在注射诱导胰腺炎后第4,12,24,36小时共4个时点分批处死大鼠,对胰腺组织进行组织病理学评分,并用酶联免疫吸附法(ELISA)检测血清淀粉酶水平、血清IL-6水平.结果:A组各时点IL-6、血清淀粉酶水平以及胰腺组织病理学评分较C组明显增高(P＜0.05或P＜0.01);其中,胰腺组织病理学评分及血清淀粉酶升高以第24,36小时最为显著;IL-6在4 h达高峰;I组组织病理学评分(除第4小时外)、血清淀粉酶及IL-6各时点值均显著低于A组(P＜0.05或P＜0.01).结论:早期应用重组人IL-10可以抑制炎症细胞因子IL-6释放,降低炎症反应的严重程度,减轻实验性胰腺炎的病理损害.%Objective: To investigate the effects of recombinant human interleukin (IL)-10 on serum inter-leukin-6 levels in L-arginine-induced acute necrotizing pancreatitis rats. Methods: Ninty-two Sprague-Daw-ley rats were randomly divided into three groups. Group A ( n =36) and Group Ⅰ ( n =32) received three intraperitoneal injection of 6 % L-arginine (3 × 1.0 mg/g) at hourly intervals. Group Ⅰ -was also treated with 10 000 unites of intraperitioneal recombinant human IL-10 at the 2nd, 5th and 8th hours after the last injection of L-arginine. Group C ( n =24) received saline alone. Rats were killed at the 4th, 12th, 24th and 36th hours after the last L-arginine injection. Serum interleukin-1β and amylase were assayed. Pancreatic tissues -were fixed in formalin. Histopathological score -was recorded according to the edema, inflammation and necrosis of the tissues in three groups. Results: Compared -with group C, serum amylase and Interleu-kin-6 , pancreatic histopathological scores in group A increased significantly after L-arginine injection ( P < 0. 05 or P <0. 01). Compared with group A , the levels of serum amylase, Interleukin-6 in group I were significantly decreased at various time points ( P <0. 05 or P <0. 01). Pancreatic histopathological scores in group I -were decreased as compared -with group A at the 12th, 24th, 36th hours ( P <0. 05 or P < 0. 01). Conclusion: IL-10 attenuates the severity of experimental acute necrotizing pancreatitis by inhibiting the release of interleukin-6.
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