Objective:To investigate the clinical assessment of thyroid carcinoma in acoustic pulse radiation imaging and the correlation analysis of osteopontin expression .Methods:Totally 71 lesions of 60 patients with thyroid carcinoma (TC) in Hainan were selected .All patients were graded by TI-RADS grading and confirmed by pathology after operation .The expression of OPN was detected by immunohistochemical S-P method .The expression of OPN mRNA was detected by fluorescence quantitative polymerase chain reaction (FQ-PCR) .The correlation between OPN expression and acoustic pulse radiation imaging was analyzed .Results:① 60 patients (933.3% ) with primary thyroid cancer and four (66.7% ) patients with secondary thyroid cancer were found in 60 patients with TC .Thirty-seven patients (616.7% According to the pathological features ,32 cases (533.3% ) had papillary thyroid carcinoma ,diffuse sclerosis 19 cases of cancer (316.7% ) ,follicular carcinoma in 9 cases (150.0% ) .②The expression of OPN mRNA in 37 cases of calcified thyroid carcinoma was significantly higher than that in non-calcified thyroid carcinoma (P<00.5) ,and the increase of OPN expression was in direct proportion to the distribution of gritty calcification (P<00.5) .Conclution :The distribution of calcified microsomes in thyroid cancer was positively correlated with the expression of OPN ,suggesting that enhanced OPN expression may be related to the formation of sand bodies ,the increased hardness of nodules and the increased malignancy .Acoustic pulse radiation imaging technology for early diagnosis of thyroid cancer and prognosis of thyroid cancer was of great significance .%目的:探讨甲状腺癌在声脉冲辐射力成像的临床评估及骨桥蛋白表达的相关性分析.方法:选取海南省甲状腺癌(thyroid carcinoma,TC)患者60例共71个病灶,所有病例均采用TI-RADS分级标准分级,并经手术后病理证实确定诊断.采用免疫组织化学S-P法检测O PN水平表达,荧光定量聚合酶链反应(FQ-PCR)测定OPN mRNA含量,并对声脉冲辐射力成像与OPN表达的进行相关性分析.结果:60例T C患者中原发性甲状腺癌56例(933.3%),继发性甲状腺癌4例(66.7%);其中存在砂粒样结节钙化型37例,占616.7%;采用T I-RADS分级,3级5例(83.3%),4级37例(616.7%),5级18例(30.00%);根据病理学特征分型:甲状腺乳头性癌32例(53.33%),弥漫硬化性癌19例(316.7%),滤泡性癌9例(150.0%).影像表现中37例钙化型甲状腺癌O PN m RN A的含量明显高于非钙化型甲状腺癌(P<00.5),且OPN表达水平升高与砂粒样钙化分布情况成正比趋势(P<00.5).结论:甲状腺癌钙化型微粒体的分布与O PN的表达水平呈正相关趋势,提示O PN表达增强可能与砂粒体的形成、结节的硬度增加、恶性度升高相关联.声脉冲辐射力成像技术对甲状腺癌的早期诊断及预测甲状腺癌的预后具有重要意义.
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