目的：：研究重组人血小板源生长因子(rhPDGF)凝胶对糖尿病溃疡大鼠创面愈合及创面中炎症反应、血管新生的影响。方法：选择清洁级雄性 Wistar 大鼠作为研究对象,制作糖尿病溃疡模型后随即分为模型组、低剂量组和高剂量组,分别给予空白凝胶、30μg/g rhPDGF 凝胶剂、100μg/g rhPDGF 凝胶剂干预。干预后4、6、8、10 d 时,观察溃疡创面的面积；6、10天时,收集创面组织并检测炎症反应分子、血管新生分子的表达量。结果：干预后4、6、8、10 d 时,低剂量和高剂量组大鼠的溃疡创面面积均显著小于模型组,高剂量组大鼠的溃疡创面面积均显著小于低剂量组；干预后6、10 d 时,低剂量和高剂量组大鼠溃疡创面中 TNF-α、IL-2的含量均显著低于模型组,VEGF、eNOS、NO 的含量均显著高于模型组；高剂量组大鼠溃疡创面中 TNF-α、IL-2的含量均显著低于低剂量组,VEGF、eNOS、NO 的含量均显著高于低剂量组。结论：rhPDGF 凝胶对糖尿病溃疡大鼠的创面愈合具有促进作用,同时能够抑制炎症反应、促进血管新生。%[ABSTRACT]Objective:To study the effect of recombinant human platelet-derived growth factor (rhPDGF)gel on wound healing as well as inflammation and angiogenesis in the wound of rats with diabetes ulcer.Methods:Clean male Wistar rats were selected as the research objects,made into diabetes ulcer models and then divided into model group,low dose group and high dose group who received blank gel,30 μg/g rhPDGF gel and 100 μg/g rhPDGF gel respectively for intervention.4 days, 6 days,8 days and 10 days after intervention,the area of ulcer wound was observed;6 days and 10 days after intervention, wound tissue was collected to detect the expression of inflammation and angiogenesis molecules.Results:4 days,6 days,8 days and 10 days after intervention,ulcer wound area of low dose group and high dose group were significantly less than those of model group,and ulcer wound area of high dose group were significantly less than those of low dose group;6 days and 10 days after intervention,TNF-αand IL-2 levels in ulcer wounds of low dose group and high dose group were significantly lower than those of model group while VEGF,eNOS and NO levels were significantly higher than those of model group;TNF-αand IL-2 levels in ulcer wounds of high dose group were significantly lower than those of low dose group while VEGF,eNOS and NO levels were significantly higher than those of low dose group.Conclusion:rhPDGF gel has promoting effect on the wound healing in rats with diabetes ulcer,and can also inhibit inflammation and promote angiogenesis.
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